TABLE 1.
Characteristics of MAbs recognizing linear epitopes
| Assay or parameter | Characteristic of MAb
|
||
|---|---|---|---|
| A1 | A69 | B1 | |
| Specificity in Western blot analysesa | VP1 | VP1, VP2 | VP1, VP2, VP3 |
| Specificity in immunoprecipitationsa | VP1, complexes with VP1 | VP1, VP2, complexes containing VP1 or VP2 | VP1, VP2, VP3 |
| IgG subclassb | IgG2a | IgG1 | IgG1 |
| Recognized epitope sequencec | KRVLEPLGL | LNFGQTGDADSV | IGTRYLTR |
a
Data from Wistuba et al. (52).
b
Determined using the Amersham MAb isotyping kit.
c
Determined by gene fragment phage display library and peptide scanning and verified by peptide competition as described in Materials and Methods.