FIG. 2.
Schematic representation of the pleiotropic effect locus (pel) (A) and nucleotide sequence of part of pClone2 (B). The filled arrow represents the putative pel-sagA protein-coding region. The gray arrow below the line represents the putative pel promoter. The vertical lines indicate the insertion site of Tn917 and potential ribosome binding site (RBS) and transcription terminator. The hatched box represents the pel mRNA. The size of this mRNA is consistent with the Northern blot analysis of pel (Fig. 4). The lowercase region is Tn917 sequence derived from pClone2. The 32 nucleotides in bold are 100% homologous to the published sagA sequence (6). The underlined sequence is part of the putative pel promoter. The remainder of the streptococcal sequence was 93% identical to the corresponding sequence in the M1 streptococcal genome database. The sequence shown is upstream of the pel gene.