TABLE 3.
Analysis of the phenotypes of representative opacity factor-positive and -negative GAS isolates transduced with phage A25 containing a disrupted pel regiona
| Phenotype | % Expression in:
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| CS101 (M49)
|
NZ131 (M49)
|
1881 (M1)
|
SF370 (M1)
|
MGAS 166s (M1)
|
||||||
| Wild type | Transductant | Wild type | Transductant | Wild type | Transductant | Wild type | Transductant | Wild type | Transductant | |
| SK | 100 | <10 | 100 | <10 | 100 | <10 | 100 | <10 | 100 | <10 |
| SpeB | 100 | <10 | 100 | <10 | 100 | <10 | 100 | <10 | 100 | <10 |
| β-Hemolysis | + | − | + | − | + | − | + | − | + | − |
| Fibrinogen binding | 100 | 36 | 100 | 35 | 100 | 42 | 100 | 38 | 100 | 37 |
a
All transductants were Emr and contained the expected 6.6-kb fragment containing Tn917 (Fig. 4). Expression of each surface or secreted virulence factor in the transductant was compared to the level of expression of the wild-type isogenic pair, which was designated as 100%. +, detectable; −, nondetectable.