TABLE 1.
Clinical and laboratory features of the human H5N1 viruses used in this study
| Virus | Casea | Age (yr)/ genderab | Date of onset in 1997a | Clinical outcomea | Plaque size in MDCK cellsc | Passage historyad | MLD50 (PFU) | CLD50 (PFU) | PB2 amino acid at position 627 |
|---|---|---|---|---|---|---|---|---|---|
| Group 1 (pathogenic in mice) | |||||||||
| A/Hong Kong/156/97 | 1 | 3/M | May 9 | Died | Pinpoint (∼20%), medium (∼80%) | C2/C3 | 0.6 | 2.3 × 102 | Glue |
| A/Hong Kong/483/97 | 3 | 13/F | November 20 | Died | Large | C1/C3 | 0.3 | 1.7 × 102 | Lys |
| A/Hong Kong/485/97 | 7 | 24/F | December 4 | Critical/ discharged | Medium | C2/C2 | 1.1 | NTf | Lys |
| A/Hong Kong/491/97 | 9 | 4/M | December 10 | Discharged | Large | X/E1C1 | 11 | NT | Glu |
| A/Hong Kong/514/97 | 13 | 25/F | December 17 | Died | Large | X/C2 | 1.0 | NT | Lys/Glug |
| A/Hong Kong/516/97 | 12 | 60/F | December 16 | Died | Large | X/C3 | 5.9 | NT | Glu |
| Group 2 (less pathogenic in mice) | |||||||||
| A/Hong Kong/481/97 | 2 | 2/M | November 6 | Discharged | Pinpoint | X/C3 | 6.0 × 103 | NT | Glu |
| A/Hong Kong/482/97 | 4 | 54/M | November 24 | Died | Pinpoint | C1/C3 | 9.3 × 103 | NT | Glu |
| A/Hong Kong/486/97 | 5h | 5/F | December 7 | Discharged | Pinpoint | C2/C2 | >6.0 × 104 | 2.3 × 102 | Glu |
| A/Hong Kong/488/97 | 8h | 2/M | December 12 | Discharged | Pinpoint | C1/C2 | 4.2 × 105 | NT | Glu |
a
Information obtained from the CDC.
b
M, male; F, female.
c
Pinpoint, diameter of <0.5 mm; medium, diameter of 0.5 to 2.0 mm; large, diameter of >2.0 mm.
d
Passage history in Hong Kong/in the United States. C, MDCK cells; E, eggs; X, unknown, but most likely less than two passages in MDCK cells. The number indicates the number of passages.
e
All 21 PCR clones had Glu.
f
NT, not tested.
g
Two PCR clones had Lys and one had Glu.
h
From a familial cluster.