FIG. 9.
Double labeling of MHV-infected cells at 5 h p.i. with POL and LAMP-1. Panel A shows several structures that label both for POL (10-nm gold, arrowheads) and LAMP-1 (5-nm gold, small arrows). Panel B shows an area with extensive labeling for LAMP-1 (10-nm gold, arrowheads) that is also labeled to some extent with POL (5-nm gold, small arrows). Note especially the membrane structure in the bottom right (small star) where LAMP-1-positive membranes seem to surround membranes that label heavily for POL. In panel C, a similar area that is heavily labeled with LAMP-1 can be seen (10-nm gold, arrowhead). A strongly labeled POL (5-nm gold, small arrows) multilamellar structure seems to be surrounded by LAMP-1-positive membranes. Bars, 100 nm.