FIG. 2.
Cleavage of La protein by 3Cpro. HeLa cells were incubated for 1, 3, 5, 7, and 10 h after poliovirus infection at 37°C (lanes 2 to 6). 3C-HeLa9 cells were incubated for 5, 7, 10, and 15 h after AxCANCre infection (lanes 7 to 10). HeLa cells were incubated for 7, 12, and 23 h after Ad5 infection (lanes 12 to 14). Mock-infected cells are indicated by an “M” (lanes 1 and 11). The cells were lysed in RIPA buffer and analyzed by immunoblot with MAbs La4B6 and SW5 as described in Materials and Methods.