Skip to main content
The British Journal of Venereal Diseases logoLink to The British Journal of Venereal Diseases
. 1975 Aug;51(4):227–231. doi: 10.1136/sti.51.4.227

Application of the enzyme-linked immunosorbent assay (ELISA) in the serodiagnosis of syphilis.

J Veldkamp, A M Visser
PMCID: PMC1046555  PMID: 1098730

Abstract

The enzyme-linked immunosorbent assay (ELISA) technique, using an ultrasonicate of Treponema pallidum as antigen, has been evaluated as a serological test for syphilis. It is concluded that the test is simple, reliable, and relatively quick and that its sensitivity in all stages of syphilis is equal to the FTAABS test. Because its specificity is probably also high, ELISA might be used in future as a first-line screening test for the serodiagnosis of syphilis.

Full text

PDF
227

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BEKKER J. H., ONVLEE P. C. Experiences with the treponemal immobilization test in the Netherlands. Antonie Van Leeuwenhoek. 1955;21(3):331–336. doi: 10.1007/BF02543829. [DOI] [PubMed] [Google Scholar]
  2. Bekker J. H., de Bruijn J. H., Miller J. N. Performance and use of the Reiter protein complement-fixation (RPCF) test. Br J Vener Dis. 1966 Mar;42(1):42–43. doi: 10.1136/sti.42.1.42. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. D'ALESSANDRO G., DARDANONI L. Isolation and purification of the protein antigen of the Reiter treponeme; a study of its serologic reactions. Am J Syph Gonorrhea Vener Dis. 1953 Mar;37(2):137–150. [PubMed] [Google Scholar]
  4. Engvall E., Perlmann P. Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G. Immunochemistry. 1971 Sep;8(9):871–874. doi: 10.1016/0019-2791(71)90454-x. [DOI] [PubMed] [Google Scholar]
  5. Miller J. N., de Bruijn J. H., Bekker J. H., Onvlee P. C. The antigenic structure of Treponema pallidum, Nichols strain. I. The demonstration, nature and location of specific and shared antigens. J Immunol. 1966 Mar;96(3):450–456. [PubMed] [Google Scholar]
  6. Van Weemen B. K., Schuurs A. H.W.M. Immunoassay using antigen-enzyme conjugates. FEBS Lett. 1971 Jun 24;15(3):232–236. doi: 10.1016/0014-5793(71)80319-8. [DOI] [PubMed] [Google Scholar]

Articles from British Journal of Venereal Diseases are provided here courtesy of BMJ Publishing Group

RESOURCES