TABLE 1.
Sequences of oligonucleotide primers and probe used for amplification of the enterotoxin gene of B. fragilis
Oligonucleotide | Sequence 5′→3′ | Size of product (bp) |
---|---|---|
Outer primers | ||
RS-3 | TGA AGT TAG TGC CCA GAT GCA GG | 367 |
RS-4 | GCT CAG CGC CCA GTA TAT GAC C | 367 |
Inner primers | ||
RS-1 | TGC GGC GAA CTC GGT TAA TGC | 290 |
RS-2 | AGC TGG GTT GTA GAC ATC CCA CTG G | 290 |
Probe RS-7 | ACG CCA AAG AGG GAC TGG AAG G | 290 |