TABLE 2.
Collaboration of VCZ with PMN for inhibition of A. fumigatus mycelial growth: XTT assay
| Condition | VCZ (μg/ml) | ΔA410 (n = 4)a | % Inhibition |
Pb
|
||
|---|---|---|---|---|---|---|
| Ic | IId | IIIe | ||||
| Medium | 0.00 | 0.477 ± 0.072 | 0 | R | ||
| 0.01 | 0.388 ± 0.062 | 19 | NS | R | ||
| 0.05 | 0.324 ± 0.059 | 32 | <0.05 | R | ||
| PMN (5 × 104/well) | 0.00 | 0.391 ± 0.093 | 18 | NS | ||
| 0.01 | 0.313 ± 0.066 | 34 | <0.05 | NS | ||
| 0.05 | 0.191 ± 0.011 | 60 | <0.01 | <0.01 | ||
a
XTT metabolism by mycelial growth in 1 h at 37°C in microtest plate wells. Inoculum, 5 × 103 germlings/well. Values are means ± standard deviations.
b
R, reference value; NS, not significant (P > 0.05).
c
Metabolism of XTT by mycelia from medium (control) cultures compared with XTT metabolism by mycelia from test cultures.
d
Metabolism of XTT by mycelia from VCZ (0.01 μg/ml) cultures compared with that of PMN plus VCZ cultures.
e
Metabolism of XTT by mycelia from VCZ (0.05 μg/ml) cultures compared with that of PMN plus VCZ cultures.