TABLE 3.
Effect of G-CSF or GM-CSF on PMN inhibition of A. fumigatus hyphal growth: XTT assay
| Condition | ΔA410 (n = 4)a | % Inhi- bition |
Pb
|
|
|---|---|---|---|---|
| Ic | IId | |||
| Medium | 0.745 ± 0.049 | 0 | R | |
| PMN (5 × 104/well) | 0.663 ± 0.049 | 11 | <0.01 | R |
| PMN + G-CSF (ng/ml) | ||||
| 100 | 0.566 ± 0.047 | 24 | <0.01 | <0.05 |
| 500 | 0.445 ± 0.025 | 40 | <0.01 | <0.01 |
| PMN + GM-CSF (U/ml) | ||||
| 100 | 0.520 ± 0.038 | 30 | <0.01 | <0.01 |
| 500 | 0.489 ± 0.021 | 34 | <0.01 | <0.01 |
a
XTT metabolism in 1 h at 37°C by hyphae from 5 × 103 conidia/well in a microtest plate. Values are means ± standard deviations.
b
R, reference value.
c
Metabolism of XTT by hyphae from medium (control) cultures compared with that by hyphae of test cultures.
d
XTT metabolism by hyphae from PMN cocultures compared with that of G-CSF- or GM-CSF-treated PMN cocultures.