TABLE 4.
Collaboration of G-CSF- and GM-CSF-treated PMN with VCZ for activity against A. fumigatus hyphae: XTT assay
| Conditiona | VCZ (μg/ml) | ΔA410 (n = 4)b | % Inhi- bition |
|---|---|---|---|
| Medium | 0 | 0.271 ± 0.410 | 0 |
| 0.01 | 0.180 ± 0.033 | 34c | |
| PMN | 0 | 0.119 ± 0.019 | 56d |
| 0.01 | 0.043 ± 0.021 | 84e,f | |
| G-CSF + PMN | 0 | 0.014 ± 0.015 | 95g |
| 0.01 | 0.006 ± 0.008 | 98h | |
| GM-CSF + PMN | 0 | 0.025 ± 0.020 | 91g |
| 0.01 | 0.002 ± 0.004 | 99h |
a
Hyphae from 103 conidia (isolate 96-92) per microtest plate well incubated in medium ± VCZ or with PMN (5 × 104 per well) ± VCZ for 24 h at 37°C. G-CSF, 100 ng/ml; GM-CSF, 500 U/ml.
b
Metabolism of XTT by washed hyphae in 1 h at 37°C was measured spectrophotometrically. Values are means ± standard deviations.
c
P < 0.05, compared to medium.
d
P < 0.01, compared to medium.
e
P < 0.05, compared to untreated PMN.
f
P < 0.01, compared to VCZ alone.
g
P < 0.01, compared to untreated PMN.
h
P < 0.05, compared to PMN plus VCZ.