TABLE 5.
Collaboration of monocytes ± GM-CSF with VCZ for inhibition of hyphal growth of A. fumigatus: MTT assay
| Condition | A570 (n = 4)a | % Inhi- bition |
Pb
|
||
|---|---|---|---|---|---|
| Ic | IId | IIIe | |||
| Medium | 0.912 ± 0.471 | 0 | R | ||
| VCZ (0.5 μg/ml) | 0.380 ± 0.144 | 58 | <0.01 | ||
| Monocytes | 0.374 ± 0.097 | 59 | <0.01 | R | R |
| Monocytes + VCZ | 0.186 ± 0.096 | 79 | <0.01 | <0.01 | |
| GM-CSF (100 U/ml) | |||||
| + Monocytes | 0.149 ± 0.029 | 83 | <0.01 | R | <0.01 |
| + Monocytes + VCZ | 0.181 ± 0.063 | 80 | <0.01 | NS | |
a
Metabolism of MTT by hyphae (isolate 96-92) in 3 h at 37°C. Values are means ± standard deviations from four determinations.
b
R, reference value; NS, not significant (P > 0.05).
c
MTT metabolism by hyphae from medium (control) cultures compared with MTT metabolism by hyphae from test cultures.
d
Metabolism of MTT by hyphae from monocyte- or GM-CSF-treated monocyte cultures compared with respective MTT metabolism by hyphae from monocyte plus VCZ and GM-CSF plus monocyte plus VCZ cultures.
e
MTT metabolism by hyphae from monocyte cultures compared with that by hyphae from GM-CSF-treated monocyte cultures.