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. 2005 May;73(5):3137–3146. doi: 10.1128/IAI.73.5.3137-3146.2005

TABLE 1.

Major cell wall-associated proteins identified in the mutanolysin extracts of GAS strains NS931, NS13, and S43 by MALDI-TOF peptide mass fingerprinting analysisa

Function or pathway Spot Protein Accession no.b Molecular mass (kDa)c pIc Peptide matchd Coverage (%)e Mutanolysin extract
Immunoreactive
Biotinylated
NS931 NS13 S43 NS931 NS13 S43 NS931 NS13 S43
Glycolysis 4 Putative pyruvate kinase Q8K7A3 54.5 4.96 30 59.8 + + + + + + + +
5 Putative NADP-dependent glyceraldehyde-3-phosphate dehydrogenase Q8K707 49.5 4.96 19 56.1 + + + + + + +
11 Phosphoglycerate kinase Q8K5W7 42.0 4.86 23 70.0 + + + + + + +
17 6-Phosphofructokinase Q8P0S6 35.8 5.34 22 50.7 + + + + +
21 Fructose-bisphosphate aldolase P82486 31.1 4.87 13 50.3 + + + + + + +
27 Triosephosphate isomerase P82478 26.5 4.57 11 63.3 + + + + + + + +
28 2,3-Bisphosphoglycerate-dependent phosphoglycerate mutase Q8P0C1 26.0 5.10 19 77.1 + + + + +
69 l-Lactate dehydrogenase Q99ZN5 35.1 5.14 9 30.7 +
Carbohydrate metabolism 1 Putative transketolase Q8K670 77.5 4.98 21 42.1 + + + + + + + + +
32 Putative dTDP-4-keto-6-deoxyglucose-3,5-epimerase Q9A046 22.4 5.07 10 42.1 + + +
41 Putative lactoylglutathione lyase Q9A121 14.4 5.09 4 30.4 +
52 Putative phosphoglucomutase Q99ZH8 63.3 4.78 10 25.7 +
54 Putative phospho-sugar mutase Q878L0 48.4 4.57 8 17.7 +
60 Putative dTDP-glucose-4,6-dehydratase Q8P199 38.8 5.41 4 12.4 + +
61 Glycerol-3-phosphate dehydrogenase [NAD(P)+] P58143 36.7 5.65 12 45.9 +
67 Tagatose 1,6-diphosphate aldolase 2 Q8K5U9 36.5 4.93 15 50.5 +
Arginine degradation 12 Ornithine carbamoyltransferase, catabolic Q8P052 37.8 5.19 19 62.8 + + + + + + + +
40f Putative carbamate kinase Q8K6Q9 33.2 4.71 12 44.3 + + + + + +
Amino acid biosynthesis 14 Putative branched-chain-amino-acid aminotransferase Q8K7U5 37.2 4.90 6 21.4 + +
72 Putative glutamine synthetase Q8NZG4 50.5 5.21 8 19.6 + +
Fatty acid and phospholipid biosynthesis 47 Putative malonyl coenzyme A-acyl carrier protein transacylase Q879J3 34.5 6.02 5 19.1 + +
Pantothenate (vitamin B5) biosynthesis 19 Putative 2-dehydropantoate 2-reductase (ketopantoate reductase) Q8P1F1 33.8 4.93 6 24.8 + + +
Pyridoxine (vitamin B6) biosynthesis 36 Putative pyridoxamine-phosphate oxidase Q8K7X7 14.9 5.87 10 68.4 +
Nucleoside metabolism 26 Putative purine nucleoside phosphorylase Q878J4 28.5 4.98 12 44.4 + + + + + +
34 Uracil phosphoribosyltrans- ferase (UMP pyrophosphorylase) Q9A194 22.8 6.30 13 57.4 + +
55 GMP synthase (gluta- mine hydrolyzing) Q8K7E6 57.5 4.91 7 14.8 +
63 Adenylosuccinate synthetase Q8P2U1 47.4 5.29 10 30.7 +
Metabolic enzyme 16 Putative phosphotransacetylase Q878S0 35.9 5.08 13 43.8 + + + +
66 Putative acetoin reduc- tase Q8P1U1 26.8 4.79 9 44.5 +
68 Probable manganese-dependent inorganic pyrophosphatase Q9A1A2 33.6 4.47 7 23.8 +
Virulence factor 8 Enolase (2-phosphoglycerate dehydratase) P82479 47.2 4.74 28 65.4 + + + + + + + + +
9 Arginine deiminasei Q8K5F0 46.1 4.99 31 70.5 + + + + + + + +
13 GAPDH P50467 35.8 5.34 18 61.8 + + + + + + + +
18f Cysteine protease SpeB precursor Q93LQ2 37.3 7.21 7 22.4 + +
23 Putative C3-degrading proteinase Q99Y63 28.6 4.89 10 43.2 +
31 Superoxide dismutase (Mn) Q8P0D4 22.5 4.87 13 81.5 + + + + +
43f Pyrogenic exotoxin M (SpeM) (fragment) Q7WYA3 24.1 7.87 6 25.0 +
48fg FcrA protein precursor Q54859 45.4 6.47 6 15.4 + +
50f,g M protein Q54840 61.7 6.24 4 6.5 + +
73 M protein (fragment) Q93LJ0 27.2 5.22 6 25.0 +
74 M protein (fragment) O86065 21.0 5.41 5 21.7 +
Protein biosynthesis 7 Elongation factor Tu Q8K872 43.8 4.91 24 58.3 + + + + +
29 Peptide deformylase Q8NZB7 22.9 5.51 11 72.5 + +
33 Ribosome recycling factor Q8P274 20.5 5.68 11 64.3 + + + +
38 Elongation factor G P82477 76.4 4.83 13 22.9 + +
44 Elongation factor Ts Q8K5L1 37.3 4.86 13 45.7 + + + +
57 Probable sigma54 modulation protein (fragments) P82482 18.4 4.45 4 30.6 +
62 Seryl-tRNA synthetase Q8K635 48.1 5.17 13 38.8 +
64 Elongation factor P P82459 20.5 4.85 4 29.2 +
Protein transport 22f Putative ABC trans- porter, substrate- binding protein Q8P2K8 30.6 7.69 7 28.6 + +
39 Trigger factor Q879L7 47.1 4.39 11 27.6 + + +
53 Putative ABC trans- porter, ATP-binding protein Q99XH2 60.7 4.77 9 21.2 + +
65 Putative copper homeostasis protein (hypothetical protein) Q8K8H0 22.6 4.79 4 24.4 +
Proteolysis and peptidolysis 6 Putative dipeptidase Q8K7L6 51.4 4.81 12 33.0 + + + + + + + +
10 Putative X-His dipeptidase Q99YT8 49.1 4.74 21 46.5 +
30 Pyrrolidone-carboxylate peptidase Q8K8C4 23.2 5.64 7 38.6 + +
45 Putative methionine aminopeptidase Q8K718 31.6 4.84 5 31.5 +
Chaperone 2 Chaperone protein DnaK P95831 64.8 4.62 24 47.6 + + + + + + + +
3 60-kDa chaperonin GroEL Q8K5M5 56.9 4.75 35 69.6 + + + + + + +
Stress protein 35 Putative alkyl hydroperoxidase Q99XR7 20.5 4.65 9 58.6 + + + + +
49 Putative glutathione reductase Q8P1H3 48.9 5.66 5 11.1 + +
Transcription factor 58 Transcription elongation factor GreA Q9A1C4 17.7 4.67 7 61.3 +
Adhesin 59f,g Collagen-like protein B (fragment) Q9AGC4 46.7 4.60 4 10.8 +
Housekeeping 15 Putative alcohol dehydrogenase I Q9A1X7 35.4 4.89 6 20.7 +
24 Putative phosphoprotein phosphatase Q99YM9 27.0 4.60 19 80.9 + +
25 Adenylate kinase Q8K8X1 23.7 4.83 13 79.7 + +
Unknown function 20 Hypothetical UPF0082 protein SPy0316/SpyM3_0231/SPs16 28/spyM18_0311 Q9A1E6 25.9 4.49 6 29.0 +
37 Hypothetical phage protein spyM18_0356 Q8P2H6 25.1 5.37 4 13.6 +
42 Hypothetical phage protein spyM18_1764 Q8NZS3 22.7 4.63 3 20.2 +
46 Hypothetical protein SPy1262 Q99ZE5 19.9 4.93 9 42.5 + + +
51 Hypothetical protein SpyM3_0548 Q8K7Z2 15.4 6.05 4 21.2 +
56 Conserved hypothetical protein SPs1095 Q878P1 17.5 4.65 6 54.6 +
70 Conserved protein SpyM18_1567 Q8P050 26.6 4.67 5 28.6 +
71 Hypothetical protein (phage associated) SPs0647 Q879B2 26.8 5.10 5 21.0 +
a

Identified proteins are indicated by a plus sign.

b

Swiss-Prot or TrEMBL accession number.

c

Theoretical values obtained from Swiss-Prot or TrEMBL database.

d

Number of tryptic peptides detected by MALDI-TOF MS that could be matched to the protein.

e

Percentage of the protein sequence covered by the matched peptides.

f

Contains a putative secretion signal sequence identified by SignalP3.0 signal peptide prediction server (http://www.cbs.dtu.dk/services/SignalP/).

g

Contains a C-terminal LPXTG membrane anchor motif identified by Pfam motif search (http://pfam.wustl.edu/hmmsearch.shtml).