TABLE 3.
Construction of plasmids to express μNS-GFP fusions
| Constructa | Forward primerb (5′ to 3′) | Expressed proteinc | Size (kDa)d |
|---|---|---|---|
| pEGFP-C1-M3(471-721) | Not applicable | GFP/μNS(471-721) | 57.1 |
| pEGFP-C1-M3(561-721) | CGGAATTCGTGTAGTCTGGATATGTATTTGAGACACCAC | GFP/μNS(561-721) | 46.3 |
| pEGFP-C1-M3(614-721) | CGGAATTCGGAAGCGGCTGCCAAATGCCAAACTG | GFP/μNS(614-721) | 40.1 |
| pEGFP-C1-M3(625-721) | CGGAATTCGATGGACTTGACTCAGATGAATGGAAAGC | GFP/μNS(625-721) | 39.1 |
| pEGFP-C1-M3(695-721) | CGGAATTCGATGGCCTCCCTTCTATCAGCCACTCCT | GFP/μNS(695-721) | 31.1 |
a
Each construct was designed to express a GFP fusion including the indicated amino acid residues of μNS.
b
The EcoRI site added near the 5′ end of each is underlined.
c
In each of these proteins, GFP was fused to the N terminus of the μNS region.
d
That is, the expected size of the expressed fusion protein.