TABLE 3.
Patterns of V-DJ rearrangement in three lines which had functional RAG gene expression indicated by D-J rearrangement
| Cell line | Culture | Transcription patterna or V-DJ rearrangementb for family:
|
||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 558 | 3609 | V10 | 3660 | SM7 | VG | S107 | Q52 | 7183 | ||
| 1-2 | P | + | + | − | − | − | − | − | − | − |
| 1 | − | − | − | + | − | − | − | − | + | |
| 2 | − | + | − | + | − | − | − | + | + | |
| 3 | + | + | − | + | − | + | − | + | + | |
| AH7 | P | − | − | − | − | + | − | − | − | − |
| 1 | − | − | − | + | − | − | − | − | − | |
| 2 | − | − | − | + | − | − | − | − | − | |
| 6312 | P | + | + | + | − | − | − | − | − | − |
| 1 | − | − | − | − | − | + | − | + | + | |
| 2 | +* | + | + | + | + | + | + | + | + | |
| 3 | + | + | − | − | − | + | + | + | + | |
| 4 | +* | + | + | + | + | + | − | + | + | |
| 5 | + | + | + | − | − | − | − | + | + | |
| 6 | + | + | + | − | − | − | + | + | + | |
The germ line transcription pattern of the parental (P) line before expression of RAG protein is indicated by + or − at the top of each series.
Numbers 1 to 6 indicate subcultures positive for V-DJ rearrangement after expression of RAG protein. The families which were positive for V-DJ rearrangement in different subcultures are indicated by +, and those for which no V-DJ rearrangement was detected are indicated by −. The primer used for J558 rearrangement can also anneal to SM7 genes, although the 5′ primer for SM7 is specific for SM7. Since the two subcultures indicated by * are positive for SM7 rearrangement, the positive signal obtained with the J558 primers may represent either SM7 or J558.