FIG. 1.
Flow cytometry analysis of HFFs infected at various points in the cell cycle. HFFs were synchronized by contact inhibition and stimulated to cycle by replating at lower density. Cells were infected at the time of replating (G0) (A), 12 h postplating (G1) (B), or 24 h post-plating (S phase) (C). Samples of mock- and HCMV-infected cells were harvested at defined times p.i. (indicated above the histograms) and analyzed for DNA content by flow cytometry following staining of the DNA with propidium iodide. The number above each histogram on the left side indicates relative cell number.