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Effects of HCMV infection on mRNA levels of cyclins A, B, and E. HFFs were synchronized by contact inhibition and stimulated to cycle by replating at lower density. The cells were infected with HCMV or mock infected at the time of replating (G0). At defined times p.i., 6 × 107 cells were harvested from both mock- and HCMV-infected cell populations for the isolation of mRNA. The mRNA was then used for Northern analysis as described in Materials and Methods.
