FIG. 3.
Genotype-specific support of RNA replication by HDAg-S of genotypes I and III. Huh-7 cells were transfected with indicated constructs for the expression of genomic (left) or antigenomic (right) HDV RNA defective for HDAg production. Cells were also cotransfected with the plasmid vector pCMV3 (lanes −) or plasmids pHDAg-S·I (lanes I) and pHDAg-S·III (lanes III), which express genotype I and III HDAg, respectively. RNAs were isolated 6 days posttransfection and analyzed by Northern blotting. Hybridization probes detected antigenomic RNA (left) or genomic RNA (right).