TABLE 2.
RT-PCR for transcripts from different EBNA1 promoters in cell lines and tumors
| Cell lines and tumors | RT-PCR signala
|
|||
|---|---|---|---|---|
| Non-EBNA1-coding transcript FUU′ (Fp) | EBNA1-coding transcripts
|
|||
| FUK (Fp) | QUK (Qp) | YUK (Cp/Wp) | ||
| EBV(+) cell lines | ||||
| BL cell lines | ||||
| Rael | + | + | ++ | − |
| Akata | ++ | ++ | ++ | − |
| Chep | + | ++ | ++ | − |
| Wan | ++ | ++ | ++ | ++ |
| Wewak | + | ++ | ++ | ++ |
| Namalwa | − | ++ | ++c | ++ |
| Rael-AzaC | ++ | ++ | ++ | ++ |
| Raji | +b | ++ | ++c | ++ |
| AG876 | ++ | ++ | ++ | ++ |
| LCL | ||||
| IB4 | − | ++ | ++c | ++ |
| B95-8 | ++ | ++ | ++ | ++ |
| EBV(+) tumors | ||||
| eBL | ||||
| BL1 | +/− | − | ++ | − |
| BL2 | − | − | + | − |
| BL6 | − | − | ++ | − |
| BL7 | − | − | ++ | − |
| BL8 | − | − | ++ | − |
| BL9 | − | − | ++c | − |
| BL10 | − | − | ++ | +/− |
| PTLD | ||||
| PTLD1 | +/− | +/− | ++ | − |
| PTLD2 | − | +/− | ++ | − |
| NPC | ||||
| NPC1 | +/− | +/− | ++ | − |
| NPC2 | − | +/− | ++ | − |
| NPC3 | − | − | ++ | NA |
| NPC4 | − | − | ++ | NA |
| NL | ||||
| NK1 | − | − | ++ | − |
| NK2 | − | − | ++ | − |
| NTL | − | − | ++ | − |
a
Scored as described in Materials and Methods. NA, not available because of lack of RNA.
b
Positive for U2′ primer but not U′ primer.
c
Weakly positive or negative for QUK but positive for Q′UK.