FIG. 7.
Effect of endogenous IL-12 on CTL activity. The spleens of PR8-infected mice treated with neutralizing anti-IL-12 antibody (C17.8) or control antibody (rat IgG) were collected at 10 days postinfection, and splenocytes from individual mice were cultured with irradiated splenocytes infected with PR8 virus in vitro as stimulators. After 4 days, the cultured cells were tested as effector cells against 51Cr-labeled PR8-infected P815 target cells at the indicated effector/target cell ratios in a 51Cr release cytotoxicity assay. Experiments included four mice per experimental condition (two separate experiments). Values are means ± standard errors of samples. ∗, P < 0.05; ∗∗, P < 0.1. n.s., not significant by Student’s t test of the comparison between groups treated with normal rat Ig and with PR8 virus.