FIG. 8.
PV1(M) cre(2C) RNA is functionally exchangeable with the cre(VP1) RNA of HRV14 in the in vitro assay. The synthesis of VPgpU(pU) was measured as described in Materials and Methods, except that the template and VPg were varied, as indicated. The template was either PV1 cre(2C) RNA or HRV14 cre(VP1) RNA. Where indicated, PV1(M) VPg was replaced by HRV14 VPg (2 μg). Samples 2, 4, 6, and 8 contained 3CDpro. The top of the figure shows the autoradiography of the reaction products; below it the quantitation of the data is displayed. The amino acid sequences of PV1(M) and HRV14 VPg are shown below. Amino acid differences between PV1(M) VPg and that of HRV14 are indicated with boldface letters.