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Effect of CaPi coprecipitation of AAV on virus association with human airway epithelia. Epithelia were treated with AAV alone, AAV following pretreatment with 6 mM EGTA to disrupt tight junctions, or AAV-CaPi. Samples were applied to a nylon membrane, and AAV was detected with a 32P-labeled probe. Also shown are rinses and application of 5 × 109 particles of AAV to the membrane.
