Skip to main content
NCBI home page
Biochemical Journal logoLink to Biochemical Journal
. 1995 Dec 15;312(Pt 3):955–959. doi: 10.1042/bj3120955

Ca2+ release triggered by nicotinate adenine dinucleotide phosphate in intact sea urchin eggs.

C M Perez-Terzic 1, E N Chini 1, S S Shen 1, T P Dousa 1, D E Clapham 1
PMCID: PMC1136206  PMID: 8554544

Abstract

Nicotinate adenine dinucleotide phosphate (NAADP) was recently identified [Lee and Aarhus (1995) J. Biol. Chem. 270, 2152-2157; Chini, Beers and Dousa (1995) J. Biol. Chem. 270, 3116-3223] as a potent Ca(2+)-releasing agent in sea urchin egg homogenates. NAADP triggered Ca2+ release by a mechanism that was distinct from inositol 1,4,5-trisphosphate (InsP3)- and cyclic ADP-ribose (cADPR)-induced Ca2+ release. When NAADP was microinjected into intact sea urchin eggs it induced a dose-dependent increase in cytoplasmic free Ca2+ which was independent of the extracellular [Ca2+]. The Ca2+ waves elicited by microinjections of NAADP originated at the site of injection and swept across the cytosol. As previously found in sea urchin egg homogenates, NAADP-induced Ca2+ release in intact eggs was not blocked by heparin or by prior desensitization to InsP3 or cADPR. Thio-NADP, a specific inhibitor of the NAADP-induced Ca2+ release in sea urchin homogenates [Chini, Beers and Dousa (1995) J. Biol. Chem. 270, 3116-3223] blocked NAADP (but not InsP3 or cADPR) injection-induced Ca2+ release in intact sea urchin eggs. Finally, fertilization of sea urchin eggs abrogated subsequent NAADP-induced Ca2+ release, suggesting that the NAADP-sensitive Ca2+ pool may participate in the fertilization response. This study demonstrates that NAADP acts as a selective Ca(2+)-releasing agonist in intact cells.

Full text

PDF
955

Images in this article

957Figure 2
on p.957

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Berridge M. J. Inositol trisphosphate and calcium signalling. Nature. 1993 Jan 28;361(6410):315–325. doi: 10.1038/361315a0. [DOI] [PubMed] [Google Scholar]
  2. Buck W. R., Hoffmann E. E., Rakow T. L., Shen S. S. Synergistic calcium release in the sea urchin egg by ryanodine and cyclic ADP ribose. Dev Biol. 1994 May;163(1):1–10. doi: 10.1006/dbio.1994.1118. [DOI] [PubMed] [Google Scholar]
  3. Buck W. R., Rakow T. L., Shen S. S. Synergistic release of calcium in sea urchin eggs by caffeine and ryanodine. Exp Cell Res. 1992 Sep;202(1):59–66. doi: 10.1016/0014-4827(92)90404-v. [DOI] [PubMed] [Google Scholar]
  4. Chini E. N., Beers K. W., Dousa T. P. Nicotinate adenine dinucleotide phosphate (NAADP) triggers a specific calcium release system in sea urchin eggs. J Biol Chem. 1995 Feb 17;270(7):3216–3223. doi: 10.1074/jbc.270.7.3216. [DOI] [PubMed] [Google Scholar]
  5. Clapham D. E. Calcium signaling. Cell. 1995 Jan 27;80(2):259–268. doi: 10.1016/0092-8674(95)90408-5. [DOI] [PubMed] [Google Scholar]
  6. Dargie P. J., Agre M. C., Lee H. C. Comparison of Ca2+ mobilizing activities of cyclic ADP-ribose and inositol trisphosphate. Cell Regul. 1990 Feb;1(3):279–290. doi: 10.1091/mbc.1.3.279. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Epel D., Patton C., Wallace R. W., Cheung W. Y. Calmodulin activates NAD kinase of sea urchin eggs: an early event of fertilization. Cell. 1981 Feb;23(2):543–549. doi: 10.1016/0092-8674(81)90150-1. [DOI] [PubMed] [Google Scholar]
  8. Galione A. Cyclic ADP-ribose, the ADP-ribosyl cyclase pathway and calcium signalling. Mol Cell Endocrinol. 1994 Jan;98(2):125–131. doi: 10.1016/0303-7207(94)90130-9. [DOI] [PubMed] [Google Scholar]
  9. Galione A. Cyclic ADP-ribose: a new way to control calcium. Science. 1993 Jan 15;259(5093):325–326. doi: 10.1126/science.8380506. [DOI] [PubMed] [Google Scholar]
  10. Galione A., Lee H. C., Busa W. B. Ca(2+)-induced Ca2+ release in sea urchin egg homogenates: modulation by cyclic ADP-ribose. Science. 1991 Sep 6;253(5024):1143–1146. doi: 10.1126/science.1909457. [DOI] [PubMed] [Google Scholar]
  11. Galione A., McDougall A., Busa W. B., Willmott N., Gillot I., Whitaker M. Redundant mechanisms of calcium-induced calcium release underlying calcium waves during fertilization of sea urchin eggs. Science. 1993 Jul 16;261(5119):348–352. doi: 10.1126/science.8392748. [DOI] [PubMed] [Google Scholar]
  12. Hellmich M. R., Strumwasser F. Purification and characterization of a molluscan egg-specific NADase, a second-messenger enzyme. Cell Regul. 1991 Mar;2(3):193–202. doi: 10.1091/mbc.2.3.193. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lee H. C., Aarhus R. A derivative of NADP mobilizes calcium stores insensitive to inositol trisphosphate and cyclic ADP-ribose. J Biol Chem. 1995 Feb 3;270(5):2152–2157. doi: 10.1074/jbc.270.5.2152. [DOI] [PubMed] [Google Scholar]
  14. Lee H. C., Aarhus R., Walseth T. F. Calcium mobilization by dual receptors during fertilization of sea urchin eggs. Science. 1993 Jul 16;261(5119):352–355. doi: 10.1126/science.8392749. [DOI] [PubMed] [Google Scholar]
  15. Lee H. C., Walseth T. F., Bratt G. T., Hayes R. N., Clapper D. L. Structural determination of a cyclic metabolite of NAD+ with intracellular Ca2+-mobilizing activity. J Biol Chem. 1989 Jan 25;264(3):1608–1615. [PubMed] [Google Scholar]
  16. Shen S. S., Buck W. R. Sources of calcium in sea urchin eggs during the fertilization response. Dev Biol. 1993 May;157(1):157–169. doi: 10.1006/dbio.1993.1120. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES