Skip to main content
NCBI home page
Biochemical Journal logoLink to Biochemical Journal
. 1989 Feb 15;258(1):261–265. doi: 10.1042/bj2580261

The effect of external calcium and pH on inositol trisphosphate-mediated calcium release from cerebellum microsomal fractions.

S K Joseph 1, H L Rice 1, J R Williamson 1
PMCID: PMC1138349  PMID: 2784673

Abstract

Binding of D-myo-inositol 1,4,5-trisphosphate (InsP3) to rat cerebellum membranes has previously been shown to be stimulated by alkaline pH and inhibited by low concentrations of Ca2+ [Worley, Baraban, Suppatopone, Wilson & Snyder (1987) J. Biol. Chem. 262, 12132-12136]. In the present study, Scatchard analysis of InsP3 binding to cerebellum microsomes indicates that the effects of Ca2+ and pH are exerted through changes in the apparent affinity of the receptor without effects on maximal binding. The influence of extravesicular Ca2+ and pH on InsP3-mediated 45Ca2+ release was investigated. Extravesicular Ca2+ inhibited InsP3-mediated Ca2+ release. The inhibitory effect of Ca2+ was most marked when a sub-optimal concentration of InsP3 was used. An increase in extravesicular pH produced a decrease in the concentration of InsP3 that yielded half-maximal Ca2+ release. Regulation of the affinity of the InsP3 receptor by Ca2+ and pH can qualitatively account for the observed effects of these factors on InsP3-mediated Ca2+ release. Feedback inhibition of InsP3 binding by Ca2+ could provide a mechanism to generate Ca2+ oscillations, particularly under hormonal conditions that produce sub-optimal elevations of InsP3 concentration.

Full text

PDF
261

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ambler S. K., Poenie M., Tsien R. Y., Taylor P. Agonist-stimulated oscillations and cycling of intracellular free calcium in individual cultured muscle cells. J Biol Chem. 1988 Feb 5;263(4):1952–1959. [PubMed] [Google Scholar]
  2. Ambler S. K., Thompson B., Solski P. A., Brown J. H., Taylor P. Receptor-mediated inositol phosphate formation in relation to calcium mobilization: a comparison of two cell lines. Mol Pharmacol. 1987 Sep;32(3):376–383. [PubMed] [Google Scholar]
  3. Berridge M. J. Inositol trisphosphate and diacylglycerol: two interacting second messengers. Annu Rev Biochem. 1987;56:159–193. doi: 10.1146/annurev.bi.56.070187.001111. [DOI] [PubMed] [Google Scholar]
  4. Brass L. F., Joseph S. K. A role for inositol triphosphate in intracellular Ca2+ mobilization and granule secretion in platelets. J Biol Chem. 1985 Dec 5;260(28):15172–15179. [PubMed] [Google Scholar]
  5. Burgess G. M., Irvine R. F., Berridge M. J., McKinney J. S., Putney J. W., Jr Actions of inositol phosphates on Ca2+ pools in guinea-pig hepatocytes. Biochem J. 1984 Dec 15;224(3):741–746. doi: 10.1042/bj2240741. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Butler M., Morell P. Sidedness of phospholipid synthesis on brain membranes. J Neurochem. 1982 Jul;39(1):155–164. doi: 10.1111/j.1471-4159.1982.tb04714.x. [DOI] [PubMed] [Google Scholar]
  7. Capiod T., Field A. C., Ogden D. C., Sandford C. A. Internal perfusion of guinea-pig hepatocytes with buffered Ca2+ or inositol 1,4,5-trisphosphate mimics noradrenaline activation of K+ and Cl- conductances. FEBS Lett. 1987 Jun 15;217(2):247–252. doi: 10.1016/0014-5793(87)80672-5. [DOI] [PubMed] [Google Scholar]
  8. Chueh S. H., Gill D. L. Inositol 1,4,5-trisphosphate and guanine nucleotides activate calcium release from endoplasmic reticulum via distinct mechanisms. J Biol Chem. 1986 Oct 25;261(30):13883–13886. [PubMed] [Google Scholar]
  9. Danoff S. K., Supattapone S., Snyder S. H. Characterization of a membrane protein from brain mediating the inhibition of inositol 1,4,5-trisphosphate receptor binding by calcium. Biochem J. 1988 Sep 15;254(3):701–705. doi: 10.1042/bj2540701. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Guillemette G., Balla T., Baukal A. J., Catt K. J. Characterization of inositol 1,4,5-trisphosphate receptors and calcium mobilization in a hepatic plasma membrane fraction. J Biol Chem. 1988 Apr 5;263(10):4541–4548. [PubMed] [Google Scholar]
  11. Guillemette G., Balla T., Baukal A. J., Catt K. J. Inositol 1,4,5-trisphosphate binds to a specific receptor and releases microsomal calcium in the anterior pituitary gland. Proc Natl Acad Sci U S A. 1987 Dec;84(23):8195–8199. doi: 10.1073/pnas.84.23.8195. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hunyady L., Sarkadi B., Cragoe E. J., Jr, Spät A., Gárdos G. Activation of sodium-proton exchange is not a prerequisite for Ca2+ mobilization and aggregation in human platelets. FEBS Lett. 1987 Dec 10;225(1-2):72–76. doi: 10.1016/0014-5793(87)81133-x. [DOI] [PubMed] [Google Scholar]
  13. Jean T., Klee C. B. Calcium modulation of inositol 1,4,5-trisphosphate-induced calcium release from neuroblastoma x glioma hybrid (NG108-15) microsomes. J Biol Chem. 1986 Dec 15;261(35):16414–16420. [PubMed] [Google Scholar]
  14. Kruskal B. A., Maxfield F. R. Cytosolic free calcium increases before and oscillates during frustrated phagocytosis in macrophages. J Cell Biol. 1987 Dec;105(6 Pt 1):2685–2693. doi: 10.1083/jcb.105.6.2685. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Monck J. R., Reynolds E. E., Thomas A. P., Williamson J. R. Novel kinetics of single cell Ca2+ transients in stimulated hepatocytes and A10 cells measured using fura-2 and fluorescent videomicroscopy. J Biol Chem. 1988 Apr 5;263(10):4569–4575. [PubMed] [Google Scholar]
  16. Munson P. J., Rodbard D. Ligand: a versatile computerized approach for characterization of ligand-binding systems. Anal Biochem. 1980 Sep 1;107(1):220–239. doi: 10.1016/0003-2697(80)90515-1. [DOI] [PubMed] [Google Scholar]
  17. Neher E., Almers W. Fast calcium transients in rat peritoneal mast cells are not sufficient to trigger exocytosis. EMBO J. 1986 Jan;5(1):51–53. doi: 10.1002/j.1460-2075.1986.tb04176.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Prentki M., Glennon M. C., Thomas A. P., Morris R. L., Matschinsky F. M., Corkey B. E. Cell-specific patterns of oscillating free Ca2+ in carbamylcholine-stimulated insulinoma cells. J Biol Chem. 1988 Aug 15;263(23):11044–11047. [PubMed] [Google Scholar]
  19. Prentki M., Janjic D., Wollheim C. B. The regulation of extramitochondrial steady state free Ca2+ concentration by rat insulinoma mitochondria. J Biol Chem. 1983 Jun 25;258(12):7597–7602. [PubMed] [Google Scholar]
  20. Shah J., Cohen R. S., Pant H. C. Inositol trisphosphate-induced calcium release in brain microsomes. Brain Res. 1987 Sep 1;419(1-2):1–6. doi: 10.1016/0006-8993(87)90562-2. [DOI] [PubMed] [Google Scholar]
  21. Siffert W., Akkerman J. W. Activation of sodium-proton exchange is a prerequisite for Ca2+ mobilization in human platelets. 1987 Jan 29-Feb 4Nature. 325(6103):456–458. doi: 10.1038/325456a0. [DOI] [PubMed] [Google Scholar]
  22. Spät A., Lukács G. L., Eberhardt I., Kiesel L., Runnebaum B. Binding of inositol phosphates and induction of Ca2+ release from pituitary microsomal fractions. Biochem J. 1987 Jun 1;244(2):493–496. doi: 10.1042/bj2440493. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Supattapone S., Worley P. F., Baraban J. M., Snyder S. H. Solubilization, purification, and characterization of an inositol trisphosphate receptor. J Biol Chem. 1988 Jan 25;263(3):1530–1534. [PubMed] [Google Scholar]
  24. Woods N. M., Cuthbertson K. S., Cobbold P. H. Agonist-induced oscillations in cytoplasmic free calcium concentration in single rat hepatocytes. Cell Calcium. 1987 Feb;8(1):79–100. doi: 10.1016/0143-4160(87)90038-8. [DOI] [PubMed] [Google Scholar]
  25. Woods N. M., Cuthbertson K. S., Cobbold P. H. Phorbol-ester-induced alterations of free calcium ion transients in single rat hepatocytes. Biochem J. 1987 Sep 15;246(3):619–623. doi: 10.1042/bj2460619. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Worley P. F., Baraban J. M., Supattapone S., Wilson V. S., Snyder S. H. Characterization of inositol trisphosphate receptor binding in brain. Regulation by pH and calcium. J Biol Chem. 1987 Sep 5;262(25):12132–12136. [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES