FIG. 3.
CD14+ DC precursors are infected by DV type 2. (A) CD14+ DC precursors (preDC) and CD14+ precursor-derived DC (preDC>DC) were incubated with UV-irradiated or live DV at different MOI, as indicated. At 2 days postinfection, the number of virions in the cell supernatant was measured by plaque assay. The data are representative of three different donors. (asterisk, undetected). (B) CD14+ DC precursors (preDC) and CD14+ precursor-derived DC (preDC>DC) or CD34+ progenitor-derived DC (CD34>DC) were infected with UV-irradiated or live DV at MOI 5. Two days after infection, production of IL-10 (black bars) and TNF-α (grey bars) was measured in the supernatants. The data are representative of three different donors. (asterisk, undetected). (C) CD14+ DC precursors (preDC) and CD14+ precursor-derived DC (preDC>DC) were infected with UV-irradiated or live DV at different MOI, as indicated. Three days later, the cell surface expression of CD83 was assessed by flow cytometry. The data are representative of three different donors. (D) Two hours after infection of preDC with DV at an MOI of 1, the cells were cultured in GM-CSF-IL-4-TGF-β. Differentiation into CD1a+ CD14− immature DC was tested 1 and 2 days afterwards by monitoring CD1a and CD14 expression. The data are representative of three different donors.