TABLE 1.
Percentage of preDC or DC stained positive for CD14 or CD1a, respectively, without excluding dead cellsa
| Cell group | % Positive cells
|
|||
|---|---|---|---|---|
| Day 2
|
Day 5
|
|||
| UV | MOI = 5 | UV | MOI = 5 | |
| preDC | 84 | 87 | 48 | 51 |
| preDC>DC | 88 | 90 | 62 | 53 |
a
PreDC or DC derived from the CD14+ precursors (preDC > DC) were infected with DV at an MOI of 5 (MOI = 5) or with UV-inactivated DV (UV), washed, and cultured. Two days and 5 days afterwards, the percentage of cells positive for CD14 (preDC) or CD1a (DC) was measured, without electronically excluding dead cells on foreward scatter/side scatter channels.