Skip to main content
NCBI home page
Biochemical Journal logoLink to Biochemical Journal
. 1982 Mar 15;202(3):623–629. doi: 10.1042/bj2020623

Glycogen metabolism in the liver of the neonatal gsd/gsd and control (GSD/GSD) rat.

D G Clark, S D Neville, M Brinkman, O H Filsell
PMCID: PMC1158155  PMID: 6953968

Abstract

1. The metabolism of hepatic glycogen, labelled with [6-3H]glucose at day 19.5 of gestation and with 14C from [U-14C]galactose at delivery, was followed for 10 h in food-deprived gsd/gsd and control (GSD/GSD) neonatal rats. 2. In the affected pups glycogen was maintained at 12% (w/w) and there was no loss of incorporated radioactivity. 3. The 3H and 14C in glycogen from the controls were both decreased by 80%, but 14C was removed at 0--5 h and [6-3H]glucose at 5--10 h. 4. Blood glucose concentrations in the unaffected neonatal rats fell from 5.3 mM at 20 min to 1.7 mM after 10 h. In the gsd/gsd pups blood glucose concentration was decreased from 2 mM at birth to 0.3 mM at 2.5 h: it was maintained at 0.8 mM between 5 and 10 h. 5. In neonatal rats that had been dead for 10 h, hepatic glycogen was decreased by 34% in the controls and by 22% in the gsd/gsd pups. These results demonstrate that liver from the affected rats contains glycogenolytic activity, but that it is not expressed in living tissue.

Full text

PDF
623

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bashan N., Gross Y., Moses S., Gutman A. Rat liver glycogen metabolism in the perinatal period. Biochim Biophys Acta. 1979 Oct 4;587(2):145–154. doi: 10.1016/0304-4165(79)90349-0. [DOI] [PubMed] [Google Scholar]
  2. Clark D. G., Rognstad R., Katz J. Lipogenesis in rat hepatocytes. J Biol Chem. 1974 Apr 10;249(7):2028–2036. [PubMed] [Google Scholar]
  3. Clark D. G., Topping D. L., Illman R. J., Trimble R. P., Malthus R. S. A glycogen storage disease (gsd/gsd) rat: studies on lipid metabolism, lipogenesis, plasma metabolites, and bile acid secretion. Metabolism. 1980 May;29(5):415–420. doi: 10.1016/0026-0495(80)90165-1. [DOI] [PubMed] [Google Scholar]
  4. Clark D., Lee D., Rognstad R., Katz J. Futile cycles in isolated perfused rat liver and in isolated rat liver parenchymal cells. Biochem Biophys Res Commun. 1975 Nov 3;67(1):212–219. doi: 10.1016/0006-291x(75)90304-6. [DOI] [PubMed] [Google Scholar]
  5. DAWKINS M. J. GLYCOGEN SYNTHESIS AND BREAKDOWN IN FETAL AND NEWBORN RAT LIVER. Ann N Y Acad Sci. 1963 Dec 30;111:203–211. doi: 10.1111/j.1749-6632.1963.tb36960.x. [DOI] [PubMed] [Google Scholar]
  6. Devos P., Hers H. G. A molecular order in the synthesis and degradation of glycogen in the liver. Eur J Biochem. 1979 Aug 15;99(1):161–167. doi: 10.1111/j.1432-1033.1979.tb13242.x. [DOI] [PubMed] [Google Scholar]
  7. Devos P., Hers H. G. Glycogen in rat adipose tissue: sequential synthesis and random degradation. Biochem Biophys Res Commun. 1980 Aug 14;95(3):1031–1036. doi: 10.1016/0006-291x(80)91576-4. [DOI] [PubMed] [Google Scholar]
  8. Devos P., Hers H. G. Glycogen metabolism in the liver of the foetal rat. Biochem J. 1974 May;140(2):331–340. doi: 10.1042/bj1400331. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Devos P., Hers H. G. Random, presumably hydrolytic, and lysosomal glycogenolysis in the livers of rats treated with phlorizin and of newborn rats. Biochem J. 1980 Oct 15;192(1):177–181. doi: 10.1042/bj1920177. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gilbert M., Bourbon J. Turnover of liver glycogen in the rat foetus. Biochem J. 1978 Dec 15;176(3):785–789. doi: 10.1042/bj1760785. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Goodall C. M., Christie G. S., Hurley J. V. Primary epithelial tumour in the right atrium of the heart and inferior vena cava in NZR/gd inbred rats; pathology of 18 cases. J Pathol. 1975 Aug;116(4):239–251. doi: 10.1002/path.1711160407. [DOI] [PubMed] [Google Scholar]
  12. Iwamasa T., Tsuru T., Hamada T., Takeuchi T. Physicochemical and ultrastructural studies on glycogenosomes in newborn rat hepatocytes. Pathol Res Pract. 1980;167(2-4):363–373. doi: 10.1016/S0344-0338(80)80065-3. [DOI] [PubMed] [Google Scholar]
  13. Jézéquel A. M., Arakawa K., Steiner J. W. The fine structure of the normal, neonatal mouse liver. Lab Invest. 1965 Nov;14(11):1894–1930. [PubMed] [Google Scholar]
  14. Katz J., Wals P. A. Pentose cycle and reducing equivalents in rat mammary-gland slices. Biochem J. 1972 Jul;128(4):879–899. doi: 10.1042/bj1280879. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. LEJEUNE N., THINES-SEMPOUX D., HERS H. G. Tissue fractionation studies. 16. Intracellular distribution and properties of alpha-glucosidases in rat liver. Biochem J. 1963 Jan;86:16–21. doi: 10.1042/bj0860016. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Malthus R., Clark D. G., Watts C., Sneyd J. G. Glycogen-storage disease in rats, a genetically determined deficiency of liver phosphorylase kinase. Biochem J. 1980 Apr 15;188(1):99–106. doi: 10.1042/bj1880099. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Philippidis H., Hanson R. W., Reshef L., Hopgood M. F., Ballard F. J. The initial synthesis of proteins during development. Phosphoenolpyruvate carboxylase in rat liver at birth. Biochem J. 1972 Mar;126(5):1127–1134. doi: 10.1042/bj1261127. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Phillips M. J., Unakar N. J., Doornewaard G., Steiner J. W. Glycogen depletion in the newborn rat liver: an electron microscopic and electron histochemical study. J Ultrastruct Res. 1967 Apr;18(1):142–165. doi: 10.1016/s0022-5320(67)80236-3. [DOI] [PubMed] [Google Scholar]
  19. Snell K., Walker D. G. Glucose metabolism in the newborn rat. Temporal studies in vivo. Biochem J. 1973 Apr;132(4):739–752. doi: 10.1042/bj1320739. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Watts C., Gain E. R. Glycogen metabolism in the liver of the developing rat. Biochem J. 1976 Nov 15;160(2):263–270. doi: 10.1042/bj1600263. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. den Otter W., van Boxtel A. B. Relation between the glycogen content of the liver and liver weight, and its meaning for enzymology. Experientia. 1971;27(11):1271–1272. doi: 10.1007/BF02136680. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES