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. 1981 Sep 15;198(3):433–440. doi: 10.1042/bj1980433

Ca2+-induced biochemical changes in human erythrocytes and their relation to microvesiculation.

D Allan, P Thomas
PMCID: PMC1163286  PMID: 6798976

Abstract

1. Human erythrocytes were treated with Ca2+ and ionophore A23187 and measurements were made of K+ efflux, polyphosphoinositide breakdown, 1,2-diacylglycerol accumulation, phosphatidate synthesis, changes in membrane polypeptide pattern and release of microvesicles. 2. It was shown that neither transamidase-mediated protein cross-linking, proteolysis of polypeptides 2.1 (ankyrin) or 4.1, nor accumulation of diacylglycerol or phosphatidate appeared to be necessary for microvesiculation to occur. 3. Microvesicles were released only under conditions where KCl efflux leading to cell shrinkage occurred and where polyphosphoinositides were broken down. These circumstances were sufficient to cause microvesiculation only in the presence of increased intracellular concentrations of Ca2+.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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