Abstract
Flux through the tricarboxylic acid cycle was calculated from oxygen consumption in hearts perfused near the physiological work load. Activities of citrate synthase, 2-oxoglutarate dehydrogenase and succinate dehydrogenase were measured in the same hearts. Only the activities of 2-oxoglutarate dehydrogenase correlated with calculated fluxes through the cycle.
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- Alp P. R., Newsholme E. A., Zammit V. A. Activities of citrate synthase and NAD+-linked and NADP+-linked isocitrate dehydrogenase in muscle from vertebrates and invertebrates. Biochem J. 1976 Mar 15;154(3):689–700. doi: 10.1042/bj1540689. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cooney G. J., Dawson A. G. Effects of indomethacin on respiration and the alpha-glycerolphosphate shuttle in rat kidney mitochondria. Biochem Pharmacol. 1979 Apr 1;28(7):1067–1070. doi: 10.1016/0006-2952(79)90305-8. [DOI] [PubMed] [Google Scholar]
- Klingenberg M. Localization of the glycerol-phosphate dehydrogenase in the outer phase of the mitochondrial inner membrane. Eur J Biochem. 1970 Apr;13(2):247–252. doi: 10.1111/j.1432-1033.1970.tb00924.x. [DOI] [PubMed] [Google Scholar]
- Newsholme E. A., Crabtree B., Zammit V. A. Use of enzyme activities as indices of maximum rates of fuel utilization. Ciba Found Symp. 1979;(73):245–258. doi: 10.1002/9780470720561.ch14. [DOI] [PubMed] [Google Scholar]
- Ottaway J. H., McClellan J. A., Saunderson C. L. Succinic thiokinase and metabolic control. Int J Biochem. 1981;13(4):401–410. doi: 10.1016/0020-711x(81)90111-7. [DOI] [PubMed] [Google Scholar]
- Read G., Crabtree B., Smith G. H. The activities of 2-oxoglutarate dehydrogenase and pyruvate dehydrogenase in hearts and mammary glands from ruminants and non-ruminants. Biochem J. 1977 May 15;164(2):349–355. doi: 10.1042/bj1640349. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Safer B., Williamson J. R. Mitochondrial-cytosolic interactions in perfused rat heart. Role of coupled transamination in repletion of citric acid cycle intermediates. J Biol Chem. 1973 Apr 10;248(7):2570–2579. [PubMed] [Google Scholar]
- Taegtmeyer H., Hems R., Krebs H. A. Utilization of energy-providing substrates in the isolated working rat heart. Biochem J. 1980 Mar 15;186(3):701–711. doi: 10.1042/bj1860701. [DOI] [PMC free article] [PubMed] [Google Scholar]