Table 1.
A genome-wide screen identified 90 RNAi inactivations that induce lifespan extension
| Primer name | Gene name | Brief description | Functional group | Fly ortholog | Mouse ortholog | Human ortholog |
|---|---|---|---|---|---|---|
| F07 A5.1 | F07 A5.1 | Innexin, required for GAP junction | Cell structure | |||
| F35C8.6 | F35C8.6 | Profilin | Cell structure | |||
| K07H8.1 | K07H8.1 | Tubulin-specific chaperone | Cell structure | yes | yes | yes |
| W05B2.5 | W05B2.5 | Collagen | Cell structure | |||
| Y119D3_451.a | Y92C3A.1 | Cadherin domain | Cell structure | |||
| C35A11.3 | C35A11.3 | Mucin | Cell surface | |||
| F08H9.5 | F08H9.5 | C-type lectin | Cell surface | |||
| K11D12.1 | K11D12.1 | Mucin | Cell surface | |||
| ZK896.7 | ZK896.7 | C-type lectin | Cell surface | |||
| C13C4.2 | C13C4.2 | Nuclear hormone receptor | Gene expression | |||
| F15E6.1 | F15E6.1a | SET domain; PHD-finger | Gene expression | yes | ||
| F54C4.2 | F54C4.2 | Transcription elongation factor Spt4 | Gene expression | yes | yes | yes |
| R11E3.4 | R11E3.4 | SET domain | Gene expression | |||
| T01B10.4 | T01B10.4 | Nuclear hormone receptor | Gene expression | |||
| T09A5.8 | T09A5.8 | Chromo domain | Gene expression | |||
| ZC64.3 | ZC64.3 | Homeobox protein ceh-18 | Gene expression | |||
| T05A1.4 | T05A1.4 | Retrotransposon integrase | Integrase | |||
| D1054.8 | D1054.8 | Glucose/ribitol dehydrogenase | Metabolism | |||
| W09H1.5 | W09H1.5 | Zinc-containing alcohol dehydrogenase superfamily | Metabolism | yes | yes | yes |
| K08E3.5 | K08E3.5 | UTP-glucose-1-phosphate uridylyltransferase | Metabolism: galactose/starch/sucrose metabolism | yes | yes | yes |
| F57B10.3 | F57B10.3 | Phosphoglycerate mutase | Metabolism: glycolysis | |||
| F43G9.1 | F43G9.1 | Isocitrate dehydrogenase | Metabolism: TCA cycle | yes | yes | yes |
| F54H12.1 | F54H12.1 | Aconitase | Metabolism: TCA cycle | yes | yes | yes |
| F59B8.2 | F59B8.2 | Isocitrate dehydrogenase | Metabolism: TCA cycle | yes | yes | yes |
| B0261.4 | B0261.4 | Mitochondrial 39-S ribosomal protein L47 | Metabolism: ETC | yes | yes | yes |
| C33F10.12 | C33F10.12 | Mitochondrial carrier | Metabolism | |||
| C53B7.4 | C53B7.4 | Mitochondrial ATP synthase g subunit | Metabolism: ETC | yes | ||
| D2030.4 | D2030.4 | NADH:CoQ oxidoreductase subunit B18 | Metabolism: ETC | yes | yes | yes |
| F26E4.6 | F26E4.6 | Cytochrome C oxidase | Metabolism: ETC | |||
| F26E4.9 | F26E4.9 | Cytochrome C oxidase | Metabolism: ETC | yes | yes | yes |
| K04G7.4 | K04G7.4 | NADH dehydrogenase | Metabolism: ETC | yes | yes | yes |
| T20H4.5 | T20H4.5 | NADH-quinone oxidoreductase | Metabolism: ETC | yes | yes | yes |
| W09C5.8 | W09C5.8 | Cytochrome c oxidase subunit IV | Metabolism: ETC | yes | yes | yes |
| F55B11.1 | F55B11.1 | Xanthine Dehydrogenase (XDH) | Metabolism: purine metabolism | yes | yes | yes |
| Y71G12A_202.a | Y71G12B.4 | Peptidylglycine alpha-amidating monooxygenase | Neuropeptide synthesis | yes | ||
| W01A11.3 | W01A11.3 | Spectrin repeat | Nuclear migration | |||
| C08B11.7 | C08B11.7 | Ubiquitin C-terminal hydrolase | Protein turnover | yes | yes | yes |
| C16C10.7 | C16C10.7 | Ubiquitin E3 ligase | Protein turnover | yes | yes | yes |
| C51E3.7 | C51E3.7 | egl-3 prohormone convertase | Protein turnover | yes | yes | yes |
| F57C12.1 | F57C12.1 | Astacin Peptidase | Protein turnover | |||
| T05G5.10 | T05G5.10 | Eukaryotic initiation factor 5A iff-1 | Protein turnover | yes | ||
| Y71H2_380.a | Y71H2AR.2 | Papain family cysteine protease | Protein turnover | |||
| C01F6.7 | C01F6.6 | PDZ domain | Protein-protein interaction | |||
| F23B2.1 | C01F6.6 | PDZ domain | Protein-protein interaction | |||
| Y50D4A_25.a | C39F7.2 | Zinc (RING) finger, SPRY domain, Fibronectin type III domain | Protein-protein interaction | yes | yes | yes |
| F09F7.5 | F09F7.5 | P21-Rho-binding domain | Protein-protein interaction | |||
| F21H12.1 | F21H12.1 | WD domain | Protein-protein interaction | yes | yes | yes |
| Y39H10A_224.b | Y39H10A.6 | WD domain, C-terminal to LisH motif | Protein-protein interaction | yes | yes | yes |
| Y54E5A.7 | Y54E5A.7 | SPRY domain, C-terminal to LisH motif | Protein-protein interaction | yes | yes | yes |
| C36H8.1 | C36H8.1 | MSP (Major sperm protein) | Reproduction | |||
| C39E9.1 | C39E9.1 | Testis-specific protein TPX-1 like | Reproduction | |||
| Y39F10C.1 | Y39F10C.1 | Vitelline membrane outer layer protein I (VOMI) | Reproduction | |||
| B0334.8 | B0334.8 | age-1 Phosphatidylinositol 3- and 4-kinase | Signaling | yes | yes | yes |
| C01F6.1 | C01F6.1 | Copine | Signaling | |||
| C04H5.3 | C04H5.3 | Adenylate and Guanylate cyclase, natriuretic peptide receptor | Signaling | |||
| C12D8.10 | C12D8.10 | akt-1 serine/threonine kinase | Signaling | yes | yes | yes |
| C27B7.7 | C27B7.7 | Ig domain, Fibronectin type III domain | Signaling | |||
| C34B4.1 | C34B4.1 | PH domain | Signaling | yes | yes | yes |
| F35D2.3 | F35D2.3 | EGF-like | Signaling | |||
| K08D10.7 | K08D10.7 | Scramblase | Signaling | |||
| K10B4.3 | K10B4.3 | PH domain | Signaling | yes | yes | yes |
| T04A11.10 | T04A11.10 | 7TM receptor | Signaling | |||
| T05E12.4 | T05E12.4 | 7TM chemoreceptor, srw family | Signaling | |||
| T26H5.1 | T26H5.1 | 7Tm receptor | Signaling | |||
| Y46H3C_13.a | Y46H3C.1/.2b | 7TM chemoreceptor, srw family | Signaling | |||
| F40F8.5 | F40F8.5 | ABC transporter | Small molecule transport | |||
| C09B7.2 | C09B7.2 | Transposase | Transposase | |||
| C26B2.2 | C26B2.2 | unknown | ||||
| C32H11.1 | C32H11.1 | Domain of unknown function DUF141 | unknown | |||
| C32H11.5 | C32H11.5c | Domain of unknown function DUF148 | unknown | |||
| D2054.8 | E03H12.5 | unknown | ||||
| F45H10.4 | F45H10.4 | unknown | ||||
| F49F1.12 | F49F1.12 | unknown | ||||
| F55B11.3 | F55B11.5/.3d | unknown | ||||
| H06H21.8 | H06H21.8 | Domain of unknown function DUF227 | unknown | |||
| K10D2.2 | K10D2.2 | PAP/25A associated domain | unknown | |||
| R05A10.5 | R05A10.5 | unknown | ||||
| R08E3.3 | R08E3.3 | unknown | yes | yes | yes | |
| T06G6.4 | T06G6.4 | unknown | ||||
| T07A9.8 | T07A9.8 | unknown | yes | yes | yes | |
| Y43F8B.12 | Y43F8B.12 | unknown | ||||
| Y43H11_160.a | Y43h11AL.2 | unknown | yes | yes | ||
| Y46H3C_14.b | Y46H3C.6 | unknown | ||||
| Y53F4C.d | Y53F4B.23 | unknown | ||||
| Y56A3A.9 | Y56A3A.9 | unknown | ||||
| Y75B8A.13 | Y75B8A.13 | unknown | ||||
| Y75B8A.33 | Y75B8A.33 | unknown | ||||
| ZK520.2 | ZK520.2 | unknown | ||||
| C18E9.4 | C18E9.4/.10e | |||||
| Y37D8A.12 | Y37D8A.12f |
a
F15E6.1 RNAi clone may hybridize to both F15E6.1 and Y51H4A.12, two PHD domain-containing proteins.
b
Y46H3C_13.a RNAi clone spans both Y46H3C.1 and Y46H3C.2, two highly similar genes coding for 7TM chemoreceptor in the srw family.
c
C32H11.5 RNAi clone may hybridize to multiple genes (Kamath et al. 2003); C32H11.5; C32H11.6; C32H11.8; C32H11.11; all of which contain DUF148.
d
F55B11.3 RNAi clone may hybridize to both F55B11.5 and F55B11.3, two highly similar genes of unknown function.
e
C18E9.4 RNAi clone spans both C18E9.4, which encodes a NADH-ubiquinone oxidoreductase, and C18E9.10, which encodes an unknown protein.
f
Y37D8A.12 RNAi clone may hybridize to multiple genes (Kamath et al. 2003).