Table 1.
Alkylation grade and expected and found number of DNA DSBs following conversion of heat-labile sites to SSBs, produced following MMS and MNNG treatments
| MMS | MNNG | |
|---|---|---|
| DNA reactivity (nmol/gDNA and mM h) (35–38) | 900 | 84000 |
| Dose (mM h) | 1.5 | 0.005 |
| Alkylation grade (nmol/g DNA) | 1350 | 420 |
| Alkylation grade (% alkylated bases) | 0.044 (1/2, 254) | 0.014 (1/7, 245) |
| No. of alkylations in human genome | 2.7 × 106 | 8.3 ×105 |
| Expected number of DSBs | ||
| 100% conversion of alkylated bases to SSBs | 17364 ± 136.0 | 1653 ± 45.6 |
| 10% conversion of alkylated bases to SSBs | 173.4 ± 13.6 | 17.3 ± 4.0 |
| Observed DSBs | 8000 | 8000 |
| Minimal distance between events required to obtain 8000 DSBs, assuming random distribution of alkylation events | ||
| 100% conversion of alkylated bases to SSBs (bp) | 6 | 70 |
| 10% conversion of alkylated bases to SSBs (bp) | 700 | 8800 |