Skip to main content
NCBI home page
Biochemical Journal logoLink to Biochemical Journal
. 1971 Mar;121(5):817–827. doi: 10.1042/bj1210817

Identification in skeletal muscle of a distinct extracellular pool of amino acids, and its role in protein synthesis

R C Hider 1,*, E B Fern 1, D R London 1
PMCID: PMC1176670  PMID: 5113495

Abstract

1. The kinetics of radioactive labelling of extra- and intra-cellular amino acid pools and protein of the extensor digitorum longus muscle were studied after incubations with radioactive amino acids in vitro. 2. The results indicated that an extracellular pool could be defined, the contents of which were different from those of the incubation medium. 3. It was concluded that amino acids from the extracellular pool, as defined in this study, were incorporated directly into protein.

Full text

PDF
817

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Birks R. I., Davey D. F. Osmotic responses demonstrating the extracellular character of the sarcoplasmic reticulum. J Physiol. 1969 May;202(1):171–188. doi: 10.1113/jphysiol.1969.sp008802. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Block W. D., Markovs M. E., Steele B. F. Comparison between free amino acid levels in plasma deproteinated with picric acid and with sulfosalicylic acid. Proc Soc Exp Biol Med. 1966 Aug-Sep;122(4):1089–1091. doi: 10.3181/00379727-122-31333. [DOI] [PubMed] [Google Scholar]
  3. Buresová M., Gutmann E., Klicpera M. Effect of tension upon rate of incorporation of amino acids into proteins of cross-striated muscle. Experientia. 1969 Feb 15;25(2):144–145. doi: 10.1007/BF01899088. [DOI] [PubMed] [Google Scholar]
  4. COTLOVE E. Mechanism and extent of distribution of inulin and sucrose in chloride space of tissues. Am J Physiol. 1954 Mar;176(3):396–410. doi: 10.1152/ajplegacy.1954.176.3.396. [DOI] [PubMed] [Google Scholar]
  5. Cohen S. R., Stampleman P. F., Lajtha A. The temperature-dependent compartmentation of the 'extracellular space' in mouse brain slices as revealed by the markers: inulin, sucrose, d-mannitol, d-sorbitol and sulfate. Brain Res. 1970 Jul 29;21(3):419–434. doi: 10.1016/0006-8993(70)90421-x. [DOI] [PubMed] [Google Scholar]
  6. Crone C., Garlick D. The penetration of inulin, sucrose, mannitol and tritiated water from the interstitial space in muscle into the vascular system. J Physiol. 1970 Sep;210(2):387–404. doi: 10.1113/jphysiol.1970.sp009216. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. DYDYNSKA M., WILKIE D. R. THE OSMOTIC PROPERTIES OF STRIATED MUSCLE FIBERS IN HYPERTONIC SOLUTIONS. J Physiol. 1963 Nov;169:312–329. doi: 10.1113/jphysiol.1963.sp007258. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Eisenberg B., Eisenberg R. S. Selective disruption of the sarcotubular system in frog sartorius muscle. A quantitative study with exogenous peroxidase as a marker. J Cell Biol. 1968 Nov;39(2):451–467. doi: 10.1083/jcb.39.2.451. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Endo M. Entry of fluorescent dyes into the sarcotubular system of the frog muscle. J Physiol. 1966 Jul;185(1):224–238. doi: 10.1113/jphysiol.1966.sp007983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. GUROFF G., UNDENFRIEND S. The uptake of tyrosine by isolated rat diaphragm. J Biol Chem. 1960 Dec;235:3518–3522. [PubMed] [Google Scholar]
  11. Gan J. C., Jeffay H. Origins and metabolism of the intracellular amino acid pools in rat liver and muscle. Biochim Biophys Acta. 1967 Nov 28;148(2):448–459. doi: 10.1016/0304-4165(67)90141-9. [DOI] [PubMed] [Google Scholar]
  12. Garlick P. J. Turnover rate of muscle protein measured by constant intravenous infusion of 14C-glycine. Nature. 1969 Jul 5;223(5201):61–62. doi: 10.1038/223061a0. [DOI] [PubMed] [Google Scholar]
  13. Gould M. K., Rawlinson W. A. Effect of electrical stimulation and training on muscle pentose transport. Am J Physiol. 1966 Jul;211(1):141–146. doi: 10.1152/ajplegacy.1966.211.1.141. [DOI] [PubMed] [Google Scholar]
  14. HOGBEN C. A., TOCCO D. J., BRODIE B. B., SCHANKER L. S. On the mechanism of intestinal absorption of drugs. J Pharmacol Exp Ther. 1959 Apr;125(4):275–282. [PubMed] [Google Scholar]
  15. HUXLEY H. E. EVIDENCE FOR CONTINUITY BETWEEN THE CENTRAL ELEMENTS OF THE TRIADS AND EXTRACELLULAR SPACE IN FROG SARTORIUS MUSCLE. Nature. 1964 Jun 13;202:1067–1071. doi: 10.1038/2021067b0. [DOI] [PubMed] [Google Scholar]
  16. Hider R. C., Fern E. B., London D. R. Relationship between intracellular amino acids and protein synthesis in the extensor digitorum longus muscle of rats. Biochem J. 1969 Sep;114(2):171–178. doi: 10.1042/bj1140171. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Kelly D. E. The fine structure of skeletal muscle triad junctions. J Ultrastruct Res. 1969 Oct;29(1):37–49. doi: 10.1016/s0022-5320(69)80054-7. [DOI] [PubMed] [Google Scholar]
  18. Kostyo J. L. Rapid effects of growth hormone on amino acid transport and protein synthesis. Ann N Y Acad Sci. 1968 Feb 5;148(2):389–407. doi: 10.1111/j.1749-6632.1968.tb20365.x. [DOI] [PubMed] [Google Scholar]
  19. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  20. OGSTON A. G., PHELPS C. F. The partition of solutes between buffer solutions and solutions containing hyaluronic acid. Biochem J. 1961 Apr;78:827–833. doi: 10.1042/bj0780827. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. OXENDER D. L., CHRISTENSEN H. N. DISTINCT MEDIATING SYSTEMS FOR THE TRANSPORT OF NEUTRAL AMINO ACIDS BY THE EHRLICH CELL. J Biol Chem. 1963 Nov;238:3686–3699. [PubMed] [Google Scholar]
  22. Pain V. M., Manchester K. L. The influence of electrical stimulation in vitro on protein synthesis and other metabolic parameters of rat extensor digitorum longus muscle. Biochem J. 1970 Jun;118(2):209–220. doi: 10.1042/bj1180209. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Waterlow J. C., Stephen J. M. The effect of low protein diets on the turn-over rates of serums, liver and muscle proteins in the rat, measured by continuous infusion of L-[14C]lysine. Clin Sci. 1968 Oct;35(2):287–305. [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES