Figure 5.
Overexpression of DSWS. A, Detection of the endogenous DSWS protein on wild-type head sections using anti-DSWS. B, C, Immunohistochemistry on head sections from an elav-GAL4, UAS-DSWS fly. Increased amounts and vesicular localization of DSWS (arrows) are prominent after neuronal overexpression. C, Double stainings with the neuronal specific anti-elav antibody (red) confirm that the DSWS (green)-containing vesicles are within neurons. D, Immunostaining on a fly expressing the active site serine mutant form of DSWS (S985D) in neurons (using elav-GAL4) also reveals localization of the mutant protein to vesicles. E, F, Head sections from loco-GAL4, UAS-DSWS flies show strong expression in glia. F, Colocalization studies using anti-DSWS (green) and the glia-specific anti-REPO antibody (red) confirm the localization in glia. la, Lamina; me, medulla; lo, lobula; lp, lobula plate; al, antennal lobes; n, nucleus. Scale bars: A, B, D, 1 μm; C, 2 μm; E, 50 μm; F, 3 μm.