TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Source of isolation or relevant characteristic | Source or reference |
|---|---|---|
| Strains | ||
| F. nucleatum 12230 | Transtracheal isolate, working strain in the lab | 26 |
| F. nucleatum 12230-US1 | F. nucleatum 12230 ΔfadA::ermF-ermAM | This study |
| F. nucleatum ATCC 10953 | Inflamed gingiva; F. nucleatum subsp. polymorphum | P. E. Kolenbrander |
| F. nucleatum ATCC 23726 | F. nucleatum subsp. nucleatum | P. E. Kolenbrander |
| F. nucleatum ATCC 25586 | Cervicofacial lesion; F. nucleatum subsp. nucleatum | P. E. Kolenbrander |
| F. nucleatum ATCC 49256 | Periodontal pocket; F. nucleatum subsp. vincentii | P. E. Kolenbrander |
| F. nucleatum ATCC 51190 | Sinusitis in upper jaw; F. nucleatum subsp. fusiform | P. E. Kolenbrander |
| F. nucleatum PK 1594 | Periodontal pocket | P. E. Kolenbrander |
| F. nucleatum DUMC1356 | Amniotic fluid; preterm birth | 25 |
| F. nucleatum DUMC2079 | Placenta; preterm birth | 25 |
| F. nucleatum DUMC2929 | Amniotic fluid; preterm birth | 25 |
| F. nucleatum DUMC3156 | Placenta; preterm birth | G. B. Hill |
| F. nucleatum DUMC3349 | Placenta; preterm birth | 25 |
| F. gonidiaformans DUMC CF65-1 | Vaginal tract; bacterial vaginosis | 25 |
| F. gonidiaformans DUMC CF63-1 | Vaginal tract; bacterial vaginosis | 25 |
| F. naviforme DUMC CF108-1 | Vaginal tract; bacterial vaginosis | G. B. Hill |
| F. mortiferum ATCC 25557 | Maxillary abscess | P. E. Kolenbrander |
| F. periodonticum ATCC 33693 | Periodontitis | P. E. Kolenbrander |
| F. russii ATCC 25533 | Infection in a cat | P. E. Kolenbrander |
| F. simiae ATCC 33568 | Monkey dental plaque | P. E. Kolenbrander |
| F. ulcerans ATCC 49185 | Skin ulcer | P. E. Kolenbrander |
| Plasmids | ||
| pLAFR2 | Cosmid vector used for library construction (21.6 kb) | 19 |
| pYWH 1 | pLAFR2 clone containing fadA (48.2 kb) | This study |
| pCR2.1 | Cloning vector (3.9 kb) | Invitrogen |
| pYWH 401 | pCR2.1 carrying 2.4-kb fragment containing fadA (6.3 kb) | This study |
| pVA2198 | Source of the ermF-ermAM cassette (9.2 kb) | 18 |
| pYH1378 | pCR2.1 carrying ΔfadA::ermF-ermAM and flanking regions of fadA (7.1 kb) | This study |
| pRL250 | Source of sacB (14.3 kb) | 11 |
| pYH1426 | pYH1378 containing sacB (9.2 kb) | This study |