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Degenerate oligonuleotide probes used to identify the putative adhesin from an F. nucleatum genomic library
| Probe(s) | Sequenceb |
|---|---|
| FadA N-terminal sequencea | ATDAASLVGELQALDA |
| Pool 1 | GCXACXGATGCTGCTTCATTAGTXGGXGAA |
| Pool 2 | GCXACXGATGCTGCTTCTTTAGTXGGXGAA |
| Pool 3 | GCXACXGATGCTGCTAGTTTAGTXGGXGAA |
| Pool 4 | TTAGTXGGXGAATTACAGGCXTTAGATGC |
Sequence identified by protein N-terminal microsequencing.
X, A or T. The nucleotides that differ in pools 1, 2, and 3 are underlined.
