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. Author manuscript; available in PMC: 2005 Sep 13.
Published in final edited form as: J Neurochem. 2005 Jun;93(6):1444–1453. doi: 10.1111/j.1471-4159.2005.03131.x

Fig. 3.

Fig. 3

Myt1 and Myt1L interact with a single PAH domain in a mammalian two-hybrid assay in COS-1 and CG4 cells. (a) COS-1 cells and (b) CG4 cells were co-transfected with one PAH domain (Sin3BPAH2) or two PAH domains (Sin3BSF274) in the pACT vector together with either the central domain of Myt1 (Myt1502–736), the amino terminal portion of the Myt1 central domain (Myt1502–623), the carboxy terminal portion of the Myt1 central domain (Myt1617–736) or the central domain of Myt1L (Myt1L642–896) in the pBIND vector. Results are expressed as the ratio of firefly to renilla luciferase light units (RLU).Values are mean ± SD of six replicates.