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. 1982 Oct;102(2):191–206. doi: 10.1093/genetics/102.2.191

Naturally Occurring Enzyme Activity Variation in DROSOPHILA MELANOGASTER. I. Sources of Variation for 23 Enzymes

C C Laurie-Ahlberg 1, A N Wilton 1, J W Curtsinger 1, T H Emigh 1
PMCID: PMC1201933  PMID: 6818102

Abstract

The genetic component of variation of enzyme activity levels in Drosophila melanogaster was investigated by using 48 second- and 48 third-chromosome isogenic substitution lines derived from natural populations. The results confirm those of our earlier experiments with the same lines and extend them to a number of additional enzymes. All 23 enzymes show a significant genetic component to the variation in one or both sets of lines and only a small part of this variation is accounted for by variation among the lines in the amount of tissue per fly. The magnitude of line effects is, in most cases, considerably larger than the magnitude of environmental and measurement error effects, and the line effects are approximately continuous in distribution. Variation in the geographic origin and karyotype of the chromosomes generally does not contribute to the line component of variation, but allozymes provide an important source of variation for a few of the enzymes. Many of the enzymes show evidence for variation of activity modifiers that are not linked to the structural locus of the enzyme.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abraham I., Doane W. W. Genetic regulation of tissue-specific expression of amylase structural genes in Drosophila melanogaster. Proc Natl Acad Sci U S A. 1978 Sep;75(9):4446–4450. doi: 10.1073/pnas.75.9.4446. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Britten R. J., Davidson E. H. Gene regulation for higher cells: a theory. Science. 1969 Jul 25;165(3891):349–357. doi: 10.1126/science.165.3891.349. [DOI] [PubMed] [Google Scholar]
  3. Chovnick A., Gelbart W., McCarron M., Osmond B. Organization of the rosy locus in Drosophila melanogaster: evidence for a control element adjacent to the xanthine dehydrogenase structural element. Genetics. 1976 Oct;84(2):233–255. doi: 10.1093/genetics/84.2.233. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Curtsinger J. W., Laurie-Ahlberg C. C. Genetic Variability of Flight Metabolism in DROSOPHILA MELANOGASTER. I. Characterization of Power Output during Tethered Flight. Genetics. 1981 Jul;98(3):549–564. doi: 10.1093/genetics/98.3.549. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Davidson E. H., Britten R. J. Regulation of gene expression: possible role of repetitive sequences. Science. 1979 Jun 8;204(4397):1052–1059. doi: 10.1126/science.451548. [DOI] [PubMed] [Google Scholar]
  6. Dickinson W. J. A genetic locus affecting the developmental expression of an enzyme in Drosophilia melanogaster. Dev Biol. 1975 Jan;42(1):131–140. doi: 10.1016/0012-1606(75)90319-x. [DOI] [PubMed] [Google Scholar]
  7. Felder M. R. Biochemical and developmental genetics of isozymes in the mouse, Mus musculus. Isozymes Curr Top Biol Med Res. 1980;4:1–68. [PubMed] [Google Scholar]
  8. Kidwell M. G., Kidwell J. F., Sved J. A. Hybrid Dysgenesis in DROSOPHILA MELANOGASTER: A Syndrome of Aberrant Traits Including Mutation, Sterility and Male Recombination. Genetics. 1977 Aug;86(4):813–833. doi: 10.1093/genetics/86.4.813. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Laurie-Ahlberg C. C., Maroni G., Bewley G. C., Lucchesi J. C., Weir B. S. Quantitative genetic variation of enzyme activities in natural populations of Drosophila melanogaster. Proc Natl Acad Sci U S A. 1980 Feb;77(2):1073–1077. doi: 10.1073/pnas.77.2.1073. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Maroni G. Genetic control of alcohol dehydrogenase levels in Drosophila. Biochem Genet. 1978 Jun;16(5-6):509–523. doi: 10.1007/BF00484215. [DOI] [PubMed] [Google Scholar]
  11. Maroni G., Laurie-Ahlberg C. C., Adams D. A., Wilton A. N. Genetic variation in the expression of ADH in Drosophila melanogaster. Genetics. 1982 Jul-Aug;101(3-4):431–446. doi: 10.1093/genetics/101.3-4.431. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. McDonald J. F., Ayala F. J. Genetic and biochemical basis of enzyme activity variation in natural populations. I. Alcohol dehydrogenase in Drosophila melanogaster. Genetics. 1978 Jun;89(2):371–388. doi: 10.1093/genetics/89.2.371. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Thompson J. N., Jr, Ashburner M., Woodruff R. C. Presumptive control mutation for alcohol dehydrogenase in Drosophila melanogaster. Nature. 1977 Nov 24;270(5635):363–363. doi: 10.1038/270363a0. [DOI] [PubMed] [Google Scholar]
  14. Wilton A. N., Laurie-Ahlberg C. C., Emigh T. H., Curtsinger J. W. Naturally occurring enzyme activity variation in Drosophila melanogaster. II. Relationships among enzymes. Genetics. 1982 Oct;102(2):207–221. doi: 10.1093/genetics/102.2.207. [DOI] [PMC free article] [PubMed] [Google Scholar]

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