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. 1999 Aug 15;342(Pt 1):119–123.

Novel method for evaluation of the oligomeric structure of membrane proteins.

M Ramjeesingh 1, L J Huan 1, E Garami 1, C E Bear 1
PMCID: PMC1220444  PMID: 10432308

Abstract

Assessment of the quaternary structure of membrane proteins by PAGE has been problematic owing to their relatively poor solubility in non-dissociative detergents. Here we report that several membrane proteins can be readily solubilized in their native quaternary structure with the use of the detergent perfluoro-octanoic acid (PFO). Further, PFO can be used with PAGE, thereby providing a novel, accessible tool with which to assess the molecular mass of homo-multimeric protein complexes.

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Selected References

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