Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Metal content of Cys-for-Sec mutant mouse SelR and Drosophila SelR
| Sample number | Solvent | Protein concentration | Zn, equivalents |
|---|---|---|---|
| Mouse SelR as isolated | 25 mM Tris⋅HCl, 1 mM EDTA, 0.15 M NaCl, pH 6.0 | 6 mg/ml | 1.08 |
| Mouse SelR dialyzed | 10 mM Tris⋅HCl, pH 7.5 | 1.8 mg/ml | 0.33 |
| Mouse SelR dialyzed (EDTA) | 10 mM Tris⋅HCl, 4 mM EDTA, pH 7.5 | 1.7 mg/ml | 0.42 |
| DrosophilaSelR | 50 mM sodium phosphate, 300 mM NaCl, 150 mM imidazole, pH 7.0 | 1.08 mg/ml | 0.82 |
