TABLE 3.
Quantitative RT-PCR analysis of CsrRS-regulated genes
| SPy no. | Designationb | Quantity of transcripta (mean ± SEM)
|
rgg/wild-type ratioc | ||
|---|---|---|---|---|---|
| Wild-type NZ131 | speB mutant NZ131 | rgg mutant NZ131 | |||
| 0336 | csrR (CsrR) | 5.95 ± 0.8 | 7.81 ± 0.6 | 3.62 ± 0.3 | 0.6 |
| 0337 | csrS (CsrS) | 1.15 ± 0.2 | 1.23 ± 0.1 | 0.70 ± 0.0 | 0.6 |
| 0738 | sagA (SLS) | 6.32 ± 0.5 | 6.38 ± 0.5 | 26.63 ± 3.2 | 4.2 |
| 1979 | ska (SKA) | 38.39 ± 3.0 | 47.53 ± 3.9 | 58.35 ± 19.7 | 1.5 |
| 2043 | mf-1 (MF-1) | 1.00 ± 0.1 | 0.92 ± 0.2 | 4.48 ± 0.5 | 4.5 |
| 2200 | hasA (HasA) | 0.12 ± 0.0 | 0.13 ± 0.0 | 0.39 ± 0.3 | 3.3 |
| 2201 | hasB (HasB) | 0.03 ± 0.0 | 0.05 ± 0.0 | 0.07 ± 0.0 | 2.3 |
| 2202 | hasC (HasC) | 0.16 ± 0.0 | 0.18 ± 0.0 | 0.16 ± 0.0 | 1.0 |
a
The gene-specific cDNA values were normalized to the quantity of gyrA cDNA in each sample. The mean ± the standard error of the mean of two independently isolated RNA samples is given.
b
Gene designations are in italics, and the corresponding protein designations are in parentheses.
c
Quantity of gyrA-normalized cDNA in rgg mutant strain NZ131 ÷ quantity in wild-type NZ131.