FIG. 8.
Effect of combined LPS and anisomycin treatment. Macrophages were infected with L. donovani promastigotes for 18 h, washed, and then treated with increasing concentrations of anisomycin and LPS for another 18 h. (A) The numbers of parasites infected were counted microscopically. Statistical significance: *, P < 0.05 compared to untreated controls; **, P < 0.01 compared to untreated controls. (B) Supernatants were also harvested and levels of TNF-α determined by enzyme-linked immunosorbent assay. Statistical significance: *, P < 0.05 compared to untreated controls; **, P < 0.01 compared to untreated controls; ***, P < 0.005 compared to untreated controls.