Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Time-dependent activation of p38 MAPK by anisomycin. Naïve (A) and L. donovani-infected (B) macrophages were treated with anisomycin (0.1 μg/ml) for up to 6 h. Whole-cell lysates were prepared and subjected to Western blotting using antibodies specific for phospho-p38 MAPK and p38 as described in Materials and Methods. The extent of phosphorylation was quantified using a densitometer with ImageQuant software(C). Comparable results were obtained in two experiments.
