Table II.
Col-0 (C) and Ler (L) allelic state and side-chain length of the predominant glucosinolates in recombinant F3 families
F3 Family No. | Allelic State 5′ of GSL-ELONG | Allelic State at MAM1 | Allelic State at MAM-L | Allelic State 3′ of GSL-ELONG | Predominant Glucosinolate Type |
---|---|---|---|---|---|
1 | C | C | C | L | C4 |
2 | C | C | C | L | C4 |
3 | C | C | C | L | C4 |
4 | C | C | C | L | C4 |
5 | C | C | L | L | C4 |
6 | L | C | C | C | C4 |
7 | C | L | L | L | C3 |
8 | C | L | L | L | C3 |
9 | L | L | L | C | C3 |
10 | L | L | L | C | C3 |
11 | L | L | L | C | C3 |
12 | L | L | L | C | C3 |
13 | L | L | L | C | C3 |
14 | L | L | L | C | C3 |
F3 plants derived from Col-0 × CL5 F2 plants with recombination events between recMS1 and recMS9 were confirmed for homozygosity, and glucosinolates were analyzed. In general, several plants were analyzed per family. L, Plants carried the Ler-0 allele at the respective locus; C, plants harbored the Col-0 allele. C3, Predominant glucosinolates are derived from homo-Met; C4, plants accumulate glucosinolates derived from dihomo-Met. Note that plants from family 5 harbor a recombination between MAM-1 and MAM-L.