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GD inhibits the binding of PPD proteins to Dicer. GST–AGO2 (A) or GST–Hiwi (B) were transiently co-expressed with CFP–Dicer in HEK293T cells. Cells were incubated with (+) or without (−) 5 μM GD for 2 h before lysis. Proteins were recovered on glutathione–sepharose beads and visualized by immunoblotting. CFP–Dicer was detected using a polyclonal anti-GFP Ab, whereas GST–AGO2 and GST–Hiwi were detected using anti-GST Abs.
