FIG.2.
K14 and HK1-C2β transgenic mice exhibit normal skin. (A) Transgene cassettes used to generate targeted expression of C2β under control of the basal K14 promoter or suprabasal HK1 promoter. Three independent lines were generated for each construct and displayed similar phenotypes. (B) C2β protein expression in keratinocyte extracts isolated from wild-type [tg(−)], singly transgenic [tg(+)], or doubly transgenic [tg(++)] mice. Actin expression is included as a loading control. (C) Histology of wild-type (−) and transgenic skin. Note normal architecture in all cases. Expression of keratin 14 and the differentiation proteins keratin 10, involucrin, filaggrin, and loricrin in (D) wild-type and HK1-C2β and (E) K14-C2β transgenic skin (orange, differentiation marker; blue, DAPI). Bars = 100 μm.