Abstract
Multilamellar arrays of photoreceptor membrane up to 50 micrometer thick have been produced using a new method. Rhodopsin chromophore orientation in the films was studied using optical linear dichroism. The rhodopsin appears to be structurally intact and capable of photobleaching and regeneration. The production of biologically active liquid-crystal films offers a promising new approach to the study of biomembranes.
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- Applebury M. L., Zuckerman D. M., Lamola A. A., Jovin T. M. Rhodopsin. Purification and recombination with phospholipids assayed by the metarhodopsin I leads to metarhodopsin II transition. Biochemistry. 1974 Aug 13;13(17):3448–3458. doi: 10.1021/bi00714a005. [DOI] [PubMed] [Google Scholar]
- Chen Y. S., Hubbell W. L. Temperature- and light-dependent structural changes in rhodopsin-lipid membranes. Exp Eye Res. 1973 Dec 24;17(6):517–532. doi: 10.1016/0014-4835(73)90082-1. [DOI] [PubMed] [Google Scholar]
- Clark N. A., Rothschild K. J., Luippold D. A., Simon B. A. Surface-induced lamellar orientation of multilayer membrane arrays. Theoretical analysis and a new method with application to purple membrane fragments. Biophys J. 1980 Jul;31(1):65–96. doi: 10.1016/S0006-3495(80)85041-7. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gulik-Krzywicki T. Structural studies of the associations between biological membrane components. Biochim Biophys Acta. 1975 Mar 25;415(1):1–28. doi: 10.1016/0304-4157(75)90015-5. [DOI] [PubMed] [Google Scholar]
- Henderson R. The structure of the purple membrane from Halobacterium hallobium: analysis of the X-ray diffraction pattern. J Mol Biol. 1975 Apr 5;93(2):123–138. doi: 10.1016/0022-2836(75)90123-0. [DOI] [PubMed] [Google Scholar]
- Henselman R. A., Cusanovich M. A. Characterization of the recombination reaction of rhodopsin. Biochemistry. 1976 Nov 30;15(24):5321–5325. doi: 10.1021/bi00669a019. [DOI] [PubMed] [Google Scholar]
- Herbette L., Marquardt J., Scarpa A., Blasie J. K. A direct analysis of lamellar x-ray diffraction from hydrated oriented multilayers of fully functional sarcoplasmic reticulum. Biophys J. 1977 Nov;20(2):245–272. doi: 10.1016/S0006-3495(77)85547-1. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Heyn M. P., Cherry R. J., Müller U. Transient and linear dichroism studies on bacteriorhodopsin: determination of the orientation of the 568 nm all-trans retinal chromophore. J Mol Biol. 1977 Dec 15;117(3):607–620. doi: 10.1016/0022-2836(77)90060-2. [DOI] [PubMed] [Google Scholar]
- Jagger W. S., Liebman P. A. Anomalous disperison of rhodopsin in rod outer segments of the frog. J Opt Soc Am. 1976 Jan;66(1):56–59. doi: 10.1364/josa.66.000056. [DOI] [PubMed] [Google Scholar]
- Korenbrot J. I., Jones O. Linear dichroism of rhodopsin in air-water interface films. J Membr Biol. 1979;46(3):239–254. doi: 10.1007/BF01868766. [DOI] [PubMed] [Google Scholar]
- Korenbrot J. I., Pramik M. J. Formation, structure, and spectrophotometry of air-water interface films containing rhodopsin. J Membr Biol. 1977 Dec 15;37(3-4):235–262. doi: 10.1007/BF01940934. [DOI] [PubMed] [Google Scholar]
- Luzzati V., Gulik-Krzywicki T., Tardieu A. Polymorphism of lecithins. Nature. 1968 Jun 15;218(5146):1031–1034. doi: 10.1038/2181031a0. [DOI] [PubMed] [Google Scholar]
- Nagy K. Photoelectric activity of dried, oriented layers of purple membrane from Halobacterium halobium. Biochem Biophys Res Commun. 1978 Nov 14;85(1):383–390. doi: 10.1016/s0006-291x(78)80054-0. [DOI] [PubMed] [Google Scholar]
- Neugebauer D. C., Blaurock A. E. Magnetic orientation of purple membranes demonstrated by optical measurements and neutron scattering. FEBS Lett. 1977;78(1):31–35. doi: 10.1016/0014-5793(77)80266-4. [DOI] [PubMed] [Google Scholar]
- O'Brien D. F., Costa L. F., Ott R. A. Photochemical functionality of rhodopsin-phospholipid recombinant membranes. Biochemistry. 1977 Apr 5;16(7):1295–1303. doi: 10.1021/bi00626a009. [DOI] [PubMed] [Google Scholar]
- Powers L., Clark N. A. Preparation of large monodomain phospholipid bilayer smectic liquid crystals. Proc Natl Acad Sci U S A. 1975 Mar;72(3):840–843. doi: 10.1073/pnas.72.3.840. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rothschild K. J., Clark N. A. Polarized infrared spectroscopy of oriented purple membrane. Biophys J. 1979 Mar;25(3):473–487. doi: 10.1016/S0006-3495(79)85317-5. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rothschild K. J., Clark N. A., Rosen K. M., Sanches R., Hsiao T. L. Spectroscopic study of photoreceptor membrane incorporated into a multilamellar film. Biochem Biophys Res Commun. 1980 Feb 27;92(4):1266–1272. doi: 10.1016/0006-291x(80)90423-4. [DOI] [PubMed] [Google Scholar]
- Rothschild K. J., Sanches R., Hsiao T. L., Clark N. A. A spectroscopic study of rhodopsin alpha-helix orientation. Biophys J. 1980 Jul;31(1):53–64. doi: 10.1016/S0006-3495(80)85040-5. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wright W. E., Brown P. K., Wald G. The orientation of rhodopsin and other pigments in dry films. J Gen Physiol. 1972 Feb;59(2):201–212. doi: 10.1085/jgp.59.2.201. [DOI] [PMC free article] [PubMed] [Google Scholar]
- van Breugel P. J., Geurts P. H., Daemen F. J., Bonting S. L. Biochemical aspects of the visual process. XXXVIII. Effects of lateral aggregation on rhodopsin in phospholipase C-treated photoreceptor membranes. Biochim Biophys Acta. 1978 May 4;509(1):136–147. doi: 10.1016/0005-2736(78)90014-7. [DOI] [PubMed] [Google Scholar]