. 2002 Dec;70(12):7095–7104. doi: 10.1128/IAI.70.12.7095-7104.2002

TABLE 2.

Oligonucleotide primers used in this study^a

Name	Sequence (5′-3′)
1721R^b	TTAAAGACATGTCCGTCAATCC
SpeL_F^b	ATGAGAATTTTTTTACACCAG
SpeL_Fcut^b	ACCATGGGAAGAGACTATTAATATTAAGG
SpeL_R^b	CGAATTCATTTTCTTTGTTTGTGAAT
117^b	AAATGGCGTCATTTTCTCA
SpeM_F^b	ATGTTAGGAGAAAAAATG
SpeM_Fcut^b	ACCATGGTTTTCAGATGCTGTGTTGG
SpeM_R^b	CGGATCCATTTTTAGAAAAATCTTCG
SpeL_8169^b	ACATATTAACCAAACGACAATTC
SpeM_8952^b	AGTTATATCTAGCAATGTTTCTCCA
Param147^b	CCAGCGCAAGAAAGACG
Param112^b	ACATATTAACCAAACGACAATTC
Param134^b	AGTTATATCTAGCAATGTTTCTCCA
1220L^b	CAGCACCTTCCTCTTTCTCG
1220R^b	GGAAAAAGAGGGACGCAAG
G1295L^b	GGATGAGTGAATAAATCGGTAAAC
G5-358D2^b	CTTTTGACTGATAGTGACAAGCTG
SBBP4004.2^b	CTTGTGTGTGTATCGCTTGCTC
1237_Rev^b	TCATGCGGCAACACATA
SpeL_5TQ^b^,^c	TCGATGTTTTTGGTCTTGCATT
SpeL_3TQ^b^,^c	CCGGCTCCTTTTCTCTCATTAA
SpeL_probe^c	6FAM-AAAGATCAAATACATTCAATCAATGGTGGTCTCGTTAAA-TAMRA
SpeM_5TQ^b^,^c	AAAGATGTAATTAATAGAACCAATATGAAGATAACA
SpeM_3TQ^b^,^c	AATTATCATCGTCCCAGACTCTAGTTTT
SpeM_probe^c	6FAM-AGAAAATTGGCACCCAGTTAATATTTAACACGAATGA-TAMRA
SpeB_5TQ^c	CGCACTAAACCCTTCAGCTCTT
SpeB_3TQ^c	ACAGCACTTTGGTAACCGTTGA
SpeB_probe^c	6FAM-GCCTGCGCCGCCACCAGTA-TAMRA
GyrA_5TQ^c	CGACTTGTCTGAACGCCAAA
GyrA_3TQ^c	TTATCACGTTCCAAACCAGTCAA
GyrA_probe^c	6FAM-CGACGCAAACGCATATCCAAAATAGCTTG-TAMRA

Primers were designed based on the genome sequence of GAS strain MGAS8232 (serotype M18) (33).

Primers used for sequencing the speL-speM locus.

Primers and probes used for real time RT-PCR analysis (TaqMan assays). Probes were labeled at the 5′ end with the reporter dye 6-carboxyfluorescein (6FAM) and at the 3′ end with the quencher 6-carboxy-N,N,N′,N′-tetramethylrhodamine (TAMRA).