TABLE 3.
Pyrogenicity and lethality of rSpeL and rSpeM
| Toxin | Pyrogenicitya (avg temp increase in °C) | Models of lethality (no. dead/no. total animals)
|
|
|---|---|---|---|
| Endotoxin enhancementb | Miniosmotic pumpc | ||
| SpeL | 0.87 | 3/3 | 2/3 |
| SpeM | 0.67 | 3/3 | 0/3 |
| SpeC | 1.50 | 3/3 | 3/3 |
| PBS | −0.07 | 0/3 | NDd |
a
Three rabbits were used for each assay. Animals were injected intravenously with 5 μg of recombinant protein per kg at 0 h, and temperatures were recorded at 4 h. A change of 0.5°C or greater is considered to be significant in this model (29, 31).
b
Mortality was recorded for a 48-h period after injection of endotoxin.
c
Mortality was recorded for 15 days after subcutaneous implantation of a miniosmotic pump.
d
ND, not determined.