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. 1974 Apr;238(1):79–92.3. doi: 10.1113/jphysiol.1974.sp010511

The formation of synapses in regenerating mammalian striated muscle

M R Bennett, T Florin, R Woog
PMCID: PMC1330863  PMID: 4838799

Abstract

1. A histological and electrophysiological study has been made of the formation of synapses in soleus and extensor digitorum longus muscles of adult rats regenerating from minced muscle fragments.

2. In the first 8 days of regeneration, myoblast and myotubes formed within the basement membranes of degenerating myofibres.

3. Eleven- to fourteen-day regenerates contained fasicles of myotubes and myofibres and both nerves and spontaneous miniature end-plate potentials (m.e.p.p.s) were observed for the first time, although very few synapses show evoked end-plate potentials (e.p.p.s).

4. Eighteen- to twenty-six-day regenerates contained. myofibres on which only a single end-plate could be detected; 30% of these synapses had a normal amplitude—frequency distribution of m.e.p.p.s and subthreshold e.p.p.s whilst at the remainder the e.p.p. was either suprathreshold or did not occur.

5. Thirty- to sixty-day regenerates contained myofibres and end-plates with the same morphological and functional characteristics as those in normal muscle.

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Selected References

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