TABLE 1.
Phenotypes of 40-h GFP, GFP-wt-TCTP, and GFP-AA-TCTP overexpression in HEK293 cellsa
| Cell type | % of cells expressing:
|
||
|---|---|---|---|
| GFP vector | GFP-wt-TCTP | GFP-AA-TCTP | |
| Mononucleate | 85.6 ± 7.2 | 81.6 ± 7.4 | 69.6 ± 7.6 |
| Multinucleate | 5.28 ± 2.79 | 5.31 ± 0.37 | 14.6 ± 5.8 |
| Mitotic | 1.99 ± 0.72 | 1.03 ± 0.29 | 1.76 ± 1.23 |
| Condensed+cell death | 7.11 ± 5.40 | 11.8 ± 7.4 | 14.1 ± 7.8 |
| Mean no. of cells counted | 274 ± 30 | 289 ± 27 | 277 ± 33 |
a
Asynchronously growing HEK293 cells on coverslips were transfected with CMV-driven GFP, GFP-wt-TCTP, and GFP-AA-TCTP plasmids. After a 40-h incubation, the cells were fixed, permeabilized, stained with propidium iodide, and analyzed by confocal and immunofluorescence microscopy. Three independent observers testing blindly scored the phenotypes of the green fluorescent cells. Values are means ± standard deviations.